Neuraminidase, also known as Sialidase, is an enzyme that hydrolyzes terminal sialic acid residues on poly-saccharide chains. It is predominantly expressed in microorganisms such as bacteria and viruses. Cleavage of sialic acid residues by neuraminidase is believed to play several roles in infection by influenza viruses. It is thought to assist in the penetration of mucosal linings, the invasion of target cells, the elution of progeny viruses from infected cells, and the prevention of self-aggregation. Thus, neuraminidase is an important target for influenza drug development and simple, direct, and automation-ready procedures for measuring neuraminidase activity find wide applications in research and drug discovery.
Preparation instructions
Principle
In this assay, neuraminidase activity is determined by an enzymatic assay in which sialic acid released by neuraminidase results in a colorimetric (570 nm)/fluorometric (λex = 530/λem = 585 nm) product directly proportional to neuraminidase activi
1.Laura R Kuck, Rose Byrne-Nash, Jacob Gillis, Katie Bueter, Laura K Couzens, Maryna C Eichelberger, Kathy L Rowlen,. (2018-04-28) VaxArray for hemagglutinin and neuraminidase potency testing of influenza vaccines.. Vaccine, 36 ((21)):( 2937-2945 ). [PMID:29699789]
Solution Calculators
Molarity Calculator
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.