NGS Multiplex Oligos for Illumina (Index Primers Set I) - for Illumina , high purity

Item Number

N665658

• Specifications & Purity: for Illumina
• Storage Temp: Store at -20°C,Avoid repeated freezing and thawing
• Shipped In: Ice chest + Ice pads

Product Description

Product content

N665658	Component	24 T	96 T	Storage
N665658A	Adaptor for Illumina	60 μL	240 μL	-20°C. Avoid freeze/thaw cycle.
N665658B	Universal Primer for Illumina	30 μL	120 μL	-20°C. Avoid freeze/thaw cycle.
N665658C	Index 1–12 Primers for Illumina	12×10 μL	12×20 μL	-20°C. Avoid freeze/thaw cycle.

Product Introduction

This kit provides the adaptor and primer used in second-generation sequencing library construction, designed for illumina platform library construction, index primer design can meet the needs of multiple high-throughput sequencing to prepare multiple library samples, the prepared library can be used for Illumina GAIIx, HiSacnSQ, HiSeq 2500/ 2000/1000 and MiSeq sequencing sequencing platform sequencing. 2000/1000 and MiSeq sequencing.

Provide your own instruments, reagents and consumables

1. Magnetic frame: DynaMagTM-2 is recommended.

2. DNA purification recovery kit.

3. DNA building kit.

4. Anhydrous ethanol.

5. Reaction tubes: It is recommended to use low adsorption PCR tubes with 1.5 ml centrifuge tubes;

6. Tip: It is recommended to use a high quality filter tip to prevent contamination of kits and library samples.

Pre-experiment Preparation and Important Notes

1. Adaptor is a double chain structure, do not leave it above room temperature to avoid unraveling and affecting the use.

2. Please centrifuge the Index primer briefly before uncapping so that the liquid collects at the bottom of the tube to avoid cross-contamination between different primers.
Usage process

Operation steps

Please follow the protocol of the second-generation sequencing rapid DNA library building kit for the use of the second-generation sequencing multi sample connector primer kit.

Adaptor for Illumina：

5´-/5Phos/ GATCGGAAGAGCACACGTCTGAACTCCAGT*C -3´

5´-ACACTCTTTCCCTACACGACGCTCTTCCGATC*T-3´

Universal Primer for Illumina：

5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T -3´

Index 1–12 Primers for Illumina：

Index Primers for Illumina	Index
Index 1 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATCGTGATGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	ATCACGAT
Index 2 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATACATCGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	CGATGTAT
Index 3 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATGCCTAAGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	TTAGGCAT
Index 4 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATTGGTCAGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	TGACCAAT
Index 5 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATCACTGTGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	ACAGTGAT
Index 6 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATATTGGCGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	GCCAATAT
Index 7 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATGATCTGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	CAGATCAT
Index 8 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATTCAAGTGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	ACTTGAAT
Index 9 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATCTGATCGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	GATCAGAT
Index 10 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATAAGCTAGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	TAGCTTAT
Index 11 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATGTAGCCGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	GGCTACAT
Index 12 Primer for Illumina	5-CAAGCAGAAGACGGCATACGAGATTACAAGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´	CTTGTAAT