Product content Component | N665671-24T | N665671-96T | Adaptor for Illumina | 60 μl | 240 μl | Universal Primer for Illumina | 30 μl | 120 μl | Index 13-27 Primers for Illumina | 12×10 μl | 12×20 μl |
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Product Introduction This kit provides the adaptor and primer used in second-generation sequencing library construction, designed for illumina platform library construction, index primer design can meet the needs of multiple high-throughput sequencing to prepare multiple library samples, the prepared library can be used for Illumina GAIIx, HiSacnSQ, HiSeq 2500/ 2000/1000 and MiSeq sequencing sequencing platform sequencing. 2000/1000 and MiSeq sequencing. Provide your own instruments, reagents and consumables 1. Magnetic frame: DynaMagTM-2 is recommended. 2. DNA purification recovery kit. 3. DNA building kit. 4. Anhydrous ethanol. 5. Reaction tubes: It is recommended to use low adsorption PCR tubes with 1.5 ml centrifuge tubes; 6. Tip: It is recommended to use a high quality filter tip to prevent contamination of kits and library samples. Pre-experiment Preparation and Important Notes 1. Adaptor is a double chain structure, do not leave it above room temperature to avoid unraveling and affecting the use. 2. Please centrifuge the Index primer briefly before uncapping so that the liquid collects at the bottom of the tube to avoid cross-contamination between different primers.
Usage process 
Operation steps
Please follow the protocol of the second-generation sequencing rapid DNA library building kit for the use of the second-generation sequencing multi sample connector primer kit. Adaptor for Illumina： 5´-/5Phos/ GATCGGAAGAGCACACGTCTGAACTCCAGT*C -3´ 5´-ACACTCTTTCCCTACACGACGCTCTTCCGATC*T-3´ Universal Primer for Illumina： 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T -3´ Index 13–27 Primers for Illumina：
| Index Primers for Illumina | Index | Index 13 Primer for Illumina | 5-CAAGCAGAAGACGGCATACGAGATTGTTGACTGTGAC TGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | AGTCAACA | Index 14 Primer for Illumina | 5-CAAGCAGAAGACGGCATACGAGATACGGAACTGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | AGTTCCGT | Index 15 Primer for Illumina | 5-CAAGCAGAAGACGGCATACGAGATTCTGACATGTGAC TGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | ATGTCAGA | Index 16 Primer for Illumina | 5-CAAGCAGAAGACGGCATACGAGATCGGGACGGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | CCGTCCCG | Index 18 Primer for Illumina | 5-CAAGCAGAAGACGGCATACGAGATGTGCGGACGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | GTCCGCAC | Index 19 Primer for Illumina | 5-CAAGCAGAAGACGGCATACGAGATCGTTTCACGTGAC TGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | GTGAAACG | Index 20 Primer for Illumina | 5-CAAGCAGAAGACGGCATACGAGATAAGGCCACGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | GTGGCCTT | Index 21 Primer for Illumina | 5-CAAGCAGAAGACGGCATACGAGATTCCGAAACGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | GTTTCGGA | Index 22 Primer for Illumina | 5-CAAGCAGAAGACGGCATACGAGATTACGTACGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | CGTACGTA | Index 23 Primer for Illumina | 5-CAAGCAGAAGACGGCATACGAGATATCCACTCGTGAC TGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | GAGTGGAT | Index 25 Primer for Illumina | 5-CAAGCAGAAGACGGCATACGAGATATATCAGTGTGAC TGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | ACTGATAT | Index 27 Primer for Illumina | 5-CAAGCAGAAGACGGCATACGAGATAAAGGAATGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´ | ATTCCTTT |
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