NHS-Phosphine , CAS No.N638958

Item Number

N638958

• Storage Temp: Protected from light,Store at -20°C,Desiccated
• Shipped In: Ice chest + Ice pads

Product Description

Product introduction：

NHS-Phosphine is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules.

Features of NHS-Phosphine:

• Soluble—reagent easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
• Compatible—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
• Specific—NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
• Chemoselective—azide and phosphine groups do not react or interfere with components of biological samples (bioorthogonal) but conjugate to one another with high efficiency

This NHS-ester compound reacts to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The phosphine group (PPh2) reacts with azide-labeled molecules by a mechanism known asStaudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different azide-tagged compounds, this NHS-Phosphine reagent facilitates a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.

• Reactive Moiety:NHS Ester,Phosphine

• Crosslinker Type:Heterobifunctional

• Form:Powder:

• Spacer:Short(<10 Å)

• Labeling Method:Chemical Labeling

• Spacer Arm Length:5.4 Å