NIH/3T3 Whole Cell Lysate

Features and benefits
    Item Number
    cl155900
    Grouped product items
    SKUSizeAvailabilityPrice Qty
    cl155900-200μg
    200μg
    Available within 4-8 weeks(?)
    Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
    $758.90

    Mycoplasma free

    View related series
    Lysate NIH/3T3

    Basic Description

    Product NameNIH/3T3 Whole Cell Lysate
    GradeAzide Free, Carrier Free
    Product Description

    Product Description:

    The NIH/3T3 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.

    NIH/3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition. This cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays. It is therefore used for DNA transfection studies.


    Specifications & PurityCarrier Free, Azide Free, 200μg, Mycoplasma free

    Images

    NIH/3T3 Whole Cell Lysate (cl155900) - Western Blot
    All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
    Lane 1: K-562 Whole Cell Lysate (cl155898)
    Lane 2: HEK-293 Whole Cell Lysate (cl155895)
    Lane 3: A431 Whole Cell Lysate (cl155901)
    Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
    Lane 5: HT-29 Whole Cell Lysate (cl155904)
    Lysates at 20 µg per lane
    Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    NIH/3T3 Whole Cell Lysate (cl155900) - Western Blot
    All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
    Lane 1: K-562 Whole Cell Lysate (cl155898)
    Lane 2: HEK-293 Whole Cell Lysate (cl155895)
    Lane 3: A431 Whole Cell Lysate (cl155901)
    Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
    Lane 5: HT-29 Whole Cell Lysate (cl155904)
    Lysates at 20 µg per lane
    Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
    Predicted band size: 29 kDa
    Observed band size: 29 kDa

    NIH/3T3 Whole Cell Lysate (cl155900) - Western Blot
    All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
    Lane 1: K-562 Whole Cell Lysate (cl155898)
    Lane 2: HEK-293 Whole Cell Lysate (cl155895)
    Lane 3: A431 Whole Cell Lysate (cl155901)
    Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
    Lane 5: HT-29 Whole Cell Lysate (cl155904)
    Lysates at 20 µg per lane
    Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
    Predicted band size: 60 kDa
    Observed band size: 58 kDa

    Product Specifications

    FormLyophilized
    Concentration200μg, Mycoplasma free
    ReconstitutionRecommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white pow
    Storage TempStore at -80°C
    Shipped InIce chest + Ice pads
    Stability And Storage Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.

    Certificates

    Certificate of Analysis(COA)

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    1 results found

    Lot NumberCertificate TypeDateItem
    ZJ23F0800808Certificate of AnalysisAug 15, 2023 cl155900

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