The NIH/3T3 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
NIH/3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition. This cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays. It is therefore used for DNA transfection studies.
Specifications & Purity
Carrier Free, Azide Free, 200μg, Mycoplasma free
Images
NIH/3T3 Whole Cell Lysate (cl155900) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution Lane 1: K-562 Whole Cell Lysate (cl155898) Lane 2: HEK-293 Whole Cell Lysate (cl155895) Lane 3: A431 Whole Cell Lysate (cl155901) Lane 4: NIH/3T3 Whole Cell Lysate (cl155900) Lane 5: HT-29 Whole Cell Lysate (cl155904) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
NIH/3T3 Whole Cell Lysate (cl155900) - Western Blot All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution Lane 1: K-562 Whole Cell Lysate (cl155898) Lane 2: HEK-293 Whole Cell Lysate (cl155895) Lane 3: A431 Whole Cell Lysate (cl155901) Lane 4: NIH/3T3 Whole Cell Lysate (cl155900) Lane 5: HT-29 Whole Cell Lysate (cl155904) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa
NIH/3T3 Whole Cell Lysate (cl155900) - Western Blot All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution Lane 1: K-562 Whole Cell Lysate (cl155898) Lane 2: HEK-293 Whole Cell Lysate (cl155895) Lane 3: A431 Whole Cell Lysate (cl155901) Lane 4: NIH/3T3 Whole Cell Lysate (cl155900) Lane 5: HT-29 Whole Cell Lysate (cl155904) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 60 kDa Observed band size: 58 kDa
Product Specifications
Form
Lyophilized
Concentration
200μg, Mycoplasma free
Reconstitution
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white pow
Storage Temp
Store at -80°C
Shipped In
Ice chest + Ice pads
Stability And Storage
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
Certificates
Certificate of Analysis(COA)
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