Normal cynomolgus serum (NCYS) is tested for complement activity and certified to possess fully functional classical and alternative pathways of activation. NCYS was prepared from mixed gender cynomolgus monkeys. Each sample of blood was collected without anticoagulants and, after coagulation, the liquid portion was separated by centrifugation. NCYS was filtered through a 0.22 µm filter, aliquoted and frozen at -80℃. All testing for complement activity was performed on the subsequently thawed samples to guarantee that the functional activity reported is what customers will receive when the samples are thawed. Complement activity is stable for several years if NCYS is stored at -70℃ or below continuously. A slight degradation of activity may be observed following additional freeze thaw cycles.
Physical Characteristics & Structure
NCYS is a clear, straw-colored liquid containing all proteins of normal monkey serum. Although the NCYS is filtered through 0.22 µm sterile filters and is aliquoted into sterile containers, it is not packed under strictly sterile conditions and is therefore not certified as sterile.
Function
NCYS is tested for classical pathway hemolytic activity using antibody-sensitized sheep erythrocytes and for alternative pathway function using rabbit erythrocytes. The Certificate of Analysis provided with each lot gives a description of the assays and specific titers for the sera compared to a normal human serum standard.
Assays
The unit of classical pathway activity is the CH50. One CH50 unit is defined as the input of NCYS complement standard yielding 50% lysis of 1x10⁸ EA when incubated for 60 minutes at 37℃ in a total reaction volume of 1.5 mL GVB⁺⁺. The unit of alternative pathway activity is the AP50. One AP50 unit is defined as the input of NCYS complement standard yielding 50% lysis of 1.5x10⁷ rabbit erythrocytes when incubated for 30 minutes at 37℃ in a total reaction volume of 100 µL GVB° containing a final Mg-EGTA concentration of 5 mM.
Applications
NCYS is used to provide a source of monkey complement for hemolytic assays.
This NCYS has been pre-tested and certified to exhibit fully functional classical and alternative pathway complement activation.
Precautions/Toxicity/Hazards
The source is cynomolgus monkey serum, therefore precautions appropriate for handling any animal blood-derived product must be used.
Hazard Code: B WGK Germany 3
MSDS available upon request.
Specifications & Purity
>50 mg/ml, 0.22 µm filtered
Bioactivity
>75 CP50 Units/mL active classical pathway >75 AP50 Units/mL active alternative
Source
Normal Cynomolgus Monkey blood
Note
Use normal precautions for handling animal products
