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NOTCH1 Antibody - Primary antibody, specific to NOTCH1, Rabbit IgG

Features and benefits
  • Specifications & Purity: ExactAb™, Validated, Carrier Free, 1.5 mg/mL
  • Short Overview:

    pAb; Rabbit anti Human NOTCH1 Antibody; WB, IHC, ICC, IF; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
Item Number
Ab087287
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab087287-10μl (Trial Size)
Apply for free trial size(?)
Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free!
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab087287-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$198.90
Ab087287-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$309.90
Ab087287-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,319.90

pAb; Rabbit anti Human NOTCH1 Antibody; WB, IHC, ICC, IF; Unconjugated

Basic Description

Product NameNOTCH1 Antibody - Primary antibody, specific to NOTCH1, Rabbit IgG
SynonymshN1 antibody; Neurogenic locus Notch homolog protein 1 antibody; NICD antibody; NOTC1_HUMAN antibody; Notch 1 antibody; Notch 1 intracellular domain antibody; Notch homolog 1 translocation associated (Drosophila) antibody; notch1 antibody; TAN1 antibody;
Specifications & PurityExactAb™, Validated, Carrier Free, 1.5 mg/mL
Host speciesRabbit
SpecificityNOTCH1
ImmunogenA synthetic peptide derived from human Notch1 (AA 2505-2519).
Positive ControlWB: MCF-7, Jurkat, HEK293 cell lysates; IHC: Human liver; IF: HeLa.
ConjugateUnconjugated
GradeCarrier Free, ExactAb™

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration1.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InDry ice
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Application

ApplicationNotes
WB

1/500-1/1000

IHC

1/50 - 1/100

IF/ICC

1/50 - 1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0800794Certificate of AnalysisAug 14, 2023 Ab087287
ZJ23F0800793Certificate of AnalysisAug 14, 2023 Ab087287

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References

1. Nam Y, Sliz P, Song L, Aster JC, Blacklow SC.  (2006)  Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes..  Cell,  124  (5): (973-83).  [PMID:16530044]
2. Garg V, Muth AN, Ransom JF, Schluterman MK, Barnes R, King IN, Grossfeld PD, Srivastava D.  (2005)  Mutations in NOTCH1 cause aortic valve disease..  Nature,  437  (7056): (270-4).  [PMID:16025100]
3. Stittrich AB, Lehman A, Bodian DL, Ashworth J, Zong Z, Li H, Lam P, Khromykh A, Iyer RK, Vockley JG et al..  (2014)  Mutations in NOTCH1 cause Adams-Oliver syndrome..  Am J Hum Genet,  95  (3): (275-84).  [PMID:25132448]
4. Astudillo L, Da Silva TG, Wang Z, Han X, Jin K, VanWye J, Zhu X, Weaver K, Oashi T, Lopes PE et al..  (2016)  The Small Molecule IMR-1 Inhibits the Notch Transcriptional Activation Complex to Suppress Tumorigenesis..  Cancer Res,  76  (12): (3593-603).  [PMID:27197169]
5. Flesch D, Ness J, Lamers C, Dehm F, Popella S, Steri R, Ogorek I, Hieke M, Dannhardt G, Werz O, Weggen S, Schubert-Zsilavecz M..  (2015)  SAR-studies of γ-secretase modulators with PPARγ-agonistic and 5-lipoxygenase-inhibitory activity for Alzheimer's disease..  Bioorg Med Chem Lett,  25  (4): (841-846).  [PMID:25575659]
6. Shan W, Balog A, Quesnelle C, Gill P, Han WC, Norris D, Mandal S, Thiruvenkadam R, Gona KB, Thiyagarajan K, Kandula S, McGlinchey K, Menard K, Wen ML, Rose A, White R, Guarino V, Shen DR, Cvijic ME, Ranasinghe A, Dai J, Zhang Y, Wu DR, Mathur A, Rampulla R, Trainor G, Hunt JT, Vite GD, Westhouse R, Lee FY, Gavai AV..  (2015)  BMS-871: a novel orally active pan-Notch inhibitor as an anticancer agent..  Bioorg Med Chem Lett,  25  (9): (1905-1909).  [PMID:25857941]
7. Yoneyama T, Arai MA, Akamine R, Koryudzu K, Tsuchiya A, Sadhu SK, Ahmed F, Itoh M, Okamoto R, Ishibashi M..  (2017)  Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells..  J Nat Prod,  80  (9): (2453-2461).  [PMID:28817274]
8. Gómez-Galeno JE, Hurtado C, Cheng J, Yardimci C, Mercola M, Cashman JR..  (2018)  b-Annulated 1,4-dihydropyridines as Notch inhibitors..  Bioorg Med Chem Lett,  28  (20): (3363-3367).  [PMID:30201292]
9. Quaglio D, Zhdanovskaya N, Tobajas G, Cuartas V, Balducci S, Christodoulou MS, Fabrizi G, Gargantilla M, Priego EM, Carmona Pestaña Á, Passarella D, Screpanti I, Botta B, Palermo R, Mori M, Ghirga F, Pérez-Pérez MJ..  (2019)  Chalcones and Chalcone-mimetic Derivatives as Notch Inhibitors in a Model of T-cell Acute Lymphoblastic Leukemia..  ACS Med Chem Lett,  10  (4): (639-643).  [PMID:30996810]
10. Ellisen, L W LW and 6 more authors..  (1991)  TAN-1, the human homolog of the Drosophila notch gene, is broken by chromosomal translocations in T lymphoblastic neoplasms..  Cell,    (23):   [PMID:1831692]
11. Matsuno, K K and 7 more authors..  (1998)  Human deltex is a conserved regulator of Notch signalling..  Nature genetics,      [PMID:9590294]
12. Gray, G E GE and 9 more authors..  (1999)  Human ligands of the Notch receptor..  The American journal of pathology,      [PMID:10079256]
13. Zhou, S S and 6 more authors..  (2000)  SKIP, a CBF1-associated protein, interacts with the ankyrin repeat domain of NotchIC To facilitate NotchIC function..  Molecular and cellular biology,      [PMID:10713164]
14. Wu, L L and 5 more authors..  (2000)  MAML1, a human homologue of Drosophila mastermind, is a transcriptional co-activator for NOTCH receptors..  Nature genetics,      [PMID:11101851]
15. Sakamoto, Kei K and 8 more authors..  (2002)  The nephroblastoma overexpressed gene (NOV/ccn3) protein associates with Notch1 extracellular domain and inhibits myoblast differentiation via Notch signaling pathway..  The Journal of biological chemistry,    (16):   [PMID:12050162]
16. Wu, Lizi L, Sun, Tao T, Kobayashi, Karla K, Gao, Ping P and Griffin, James D JD..  (2002)  Identification of a family of mastermind-like transcriptional coactivators for mammalian notch receptors..  Molecular and cellular biology,      [PMID:12370315]
17. Vardar, Didem D, North, Christopher L CL, Sanchez-Irizarry, Cheryll C, Aster, Jon C JC and Blacklow, Stephen C SC..  (2003)  Nuclear magnetic resonance structure of a prototype Lin12-Notch repeat module from human Notch1..  Biochemistry,    (17):   [PMID:12795601]
18. Humphray, S J SJ and 147 more authors..  (2004)  DNA sequence and analysis of human chromosome 9..  Nature,    (27):   [PMID:15164053]
19. Hambleton, Sophie S and 7 more authors..  (2004)  Structural and functional properties of the human notch-1 ligand binding region..  Structure (London, England : 1993),      [PMID:15576031]
20. Ehebauer, Matthias T MT, Chirgadze, Dimitri Y DY, Hayward, Penny P, Martinez Arias, Alfonso A and Blundell, Tom L TL..  (2005)  High-resolution crystal structure of the human Notch 1 ankyrin domain..  The Biochemical journal,    (15):   [PMID:16011479]
21. Coleman, Mathew L ML and 12 more authors..  (2007)  Asparaginyl hydroxylation of the Notch ankyrin repeat domain by factor inhibiting hypoxia-inducible factor..  The Journal of biological chemistry,    (17):   [PMID:17573339]
22. Chastagner, Patricia P, Israël, Alain A and Brou, Christel C..  (2008)  AIP4/Itch regulates Notch receptor degradation in the absence of ligand..  PloS one,    (16):   [PMID:18628966]
23. Dephoure, Noah N and 6 more authors..  (2008)  A quantitative atlas of mitotic phosphorylation..  Proceedings of the National Academy of Sciences of the United States of America,    (5):   [PMID:18669648]
24. Brütsch, René R and 8 more authors..  (2010)  Integrin cytoplasmic domain-associated protein-1 attenuates sprouting angiogenesis..  Circulation research,    (3):   [PMID:20616313]
25. Mo, Jung-Soon JS and 13 more authors..  (2011)  Serum- and glucocorticoid-inducible kinase 1 (SGK1) controls Notch1 signaling by downregulation of protein stability through Fbw7 ubiquitin ligase..  Journal of cell science,    (1):   [PMID:21147854]
26. Vasquez-Del Carpio, Rodrigo R and 6 more authors..  (2011)  Assembly of a Notch transcriptional activation complex requires multimerization..  Molecular and cellular biology,      [PMID:21245387]
27. Gupta-Rossi, Neetu N and 6 more authors..  (2011)  The adaptor-associated kinase 1, AAK1, is a positive regulator of the Notch pathway..  The Journal of biological chemistry,    (27):   [PMID:21464124]
28. Lim, Seung-Oe SO and 7 more authors..  (2011)  Notch1 binds and induces degradation of Snail in hepatocellular carcinoma..  BMC biology,    (30):   [PMID:22128911]
29. Puca, Loredana L, Chastagner, Patricia P, Meas-Yedid, Vannary V, Israël, Alain A and Brou, Christel C..  (2013)  Α-arrestin 1 (ARRDC1) and β-arrestins cooperate to mediate Notch degradation in mammals..  Journal of cell science,    (1):   [PMID:23886940]
30. Boskovski, Marko T MT and 7 more authors..  (2013)  The heterotaxy gene GALNT11 glycosylates Notch to orchestrate cilia type and laterality..  Nature,    (19):   [PMID:24226769]
31. Chakrabarty, Broto and Parekh, Nita..  (2014)  Identifying tandem Ankyrin repeats in protein structures..  BMC bioinformatics,    (30):   [PMID:25547411]
32. Pinnell, Nancy N and 14 more authors..  (2015)  The PIAS-like Coactivator Zmiz1 Is a Direct and Selective Cofactor of Notch1 in T Cell Development and Leukemia..  Immunity,    (17):   [PMID:26522984]
33. Deatherage, Catherine L CL and 7 more authors..  (2017)  Structural and biochemical differences between the Notch and the amyloid precursor protein transmembrane domains..  Science advances,      [PMID:28439555]
34. Takeuchi, Hideyuki H and 6 more authors..  (2018)  Two novel protein O-glucosyltransferases that modify sites distinct from POGLUT1 and affect Notch trafficking and signaling..  Proceedings of the National Academy of Sciences of the United States of America,    (4):   [PMID:30127001]

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