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Obinutuzumab (anti-CD20), for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to MS4A1, >95%, high purity, Human IgG1, Antibody of CD20 (membrane-spanning 4-domains; subfamily A; member 1)

  • Azide Free
  • Carrier Free
  • ExactAb™
  • Low Endotoxin
  • Recombinant
  • Validated
  • ≥95%(SDS-PAGE&SEC)
  • Lot by Lot
Features and benefits
  • Short Overview:

    Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated

  • Application: ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR)
  • Species reactivity(Reacts with): Cynomolgus monkey,Human
  • Isotype: Human IgG1
    Application:
  • Animal Model
  • ELISA
  • Flow Cytometry
  • Functional Assay
Item Number
O412011
Grouped product items
SKUSizeAvailabilityPrice Qty
O412011-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$99.90
O412011-1mg
1mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$453.90
O412011-5mg
5mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$1,889.90

Purity>95% (SDS-PAGE&SEC); Endotoxin Level<1.0EU/mg; Human IgG1; CHO; ELISA, FACS, Functional assay, Animal Model; Unconjugated

Basic Description

Product NameObinutuzumab (anti-CD20), for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to MS4A1, >95%, high purity, Human IgG1
SynonymsGazyva; GA101; Gazyvaro; Afutuzumab; APY antibody; ATOPY antibody; B lymphocyte antigen CD20 antibody; B Lymphocyte Cell Surface Antigen B1 antibody; B-lymphocyte antigen CD20 antibody; B-lymphocyte cell-surface antigen B1 antibody; B-lymphocyte surface a
Specifications & PurityExactAb™, Validated, Carrier Free, Low Endotoxin, Azide Free, Recombinant, ≥95%(SDS-PAGE&SEC), Lot by Lot
SpecificityMS4A1
ApplicationELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR)
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated
Action TypeBINDING AGENT
Mechanism of actionAntibody of CD20 (membrane-spanning 4-domains; subfamily A; member 1)
Product Description

Obinutuzumab is a novel glyca-engineered type II CD20 humanized IgG1 monoclonal antibody for non-Hodgkin lymphoma.
Purity
>95% (SDS-PAGE&SEC)
Endotoxin Level
< 1.0EU/mg 

Product Properties

Antibody TypePrimary antibody
ClonalityRecombinant
IsotypeHuman IgG1
Light Chain Typekappa
SDS-PAGE27.6 kDa (Light Chain) & 52.4 kDa (Heavy Chain), under reducing conditions; 179.0 kDa, under non-reducing conditions.
Purification MethodProtein A purified
Purity>95%
SourceCHO supernatant
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -80°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -80℃ for 24 months. Upon delivery aliquot. Avoid freeze/thaw cycle.
CAS949142-50-1

Images

Obinutuzumab (anti-CD20) (O412011) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD20 (red) with Obinutuzumab (anti-CD20) (O412011). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Obinutuzumab (anti-CD20) (O412011) - SEC
The purity of Obinutuzumab (anti-CD20) (O412011) is more than 95% verified by HPLC.

Associated Targets

MS4A1 Tclin B-lymphocyte antigen CD20 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0900984Certificate of AnalysisSep 06, 2023 O412011
ZJ23F0900977Certificate of AnalysisSep 06, 2023 O412011

Related Documents

References

1. Herter S, Herting F, Mundigl O, Waldhauer I, Weinzierl T, Fauti T, Muth G, Ziegler-Landesberger D, Van Puijenbroek E, Lang S et al..  (2013)  Preclinical activity of the type II CD20 antibody GA101 (obinutuzumab) compared with rituximab and ofatumumab in vitro and in xenograft models..  Mol Cancer Ther,  12  (10): (2031-42).  [PMID:23873847]
2. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al..  (2004)  The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)..  Genome Res,  14  (10B): (2121-7).  [PMID:15489334]
3. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al..  (2004)  Complete sequencing and characterization of 21,243 full-length human cDNAs..  Nat Genet,  36  (1): (40-5).  [PMID:14702039]
4. Kuijpers TW, Bende RJ, Baars PA, Grummels A, Derks IA, Dolman KM, Beaumont T, Tedder TF, van Noesel CJ, Eldering E et al..  (2010)  CD20 deficiency in humans results in impaired T cell-independent antibody responses..  J Clin Invest,  120  (1): (214-22).  [PMID:20038800]
5. Stein R, Qu Z, Chen S, Rosario A, Shi V, Hayes M, Horak ID, Hansen HJ, Goldenberg DM.  (2004)  Characterization of a new humanized anti-CD20 monoclonal antibody, IMMU-106, and Its use in combination with the humanized anti-CD22 antibody, epratuzumab, for the therapy of non-Hodgkin's lymphoma..  Clin Cancer Res,  10  (8): (2868-78).  [PMID:15102696]
6. Le Garff-Tavernier M, Decocq J, de Romeuf C, Parizot C, Dutertre CA, Chapiro E, Davi F, Debré P, Prost JF, Teillaud JL et al..  (2011)  Analysis of CD16+CD56dim NK cells from CLL patients: evidence supporting a therapeutic strategy with optimized anti-CD20 monoclonal antibodies..  Leukemia,  25  (1): (101-9).  [PMID:20975664]
7. Li B, Xu L, Pi C, Yin Y, Xie K, Tao F, Li R, Gu H, Fang J.  (2017)  CD89-mediated recruitment of macrophages via a bispecific antibody enhances anti-tumor efficacy..  Oncoimmunology,  (1): (e1380142).  [PMID:29296544]
8. Sun LL, Ellerman D, Mathieu M, Hristopoulos M, Chen X, Li Y, Yan X, Clark R, Reyes A, Stefanich E et al..  (2015)  Anti-CD20/CD3 T cell-dependent bispecific antibody for the treatment of B cell malignancies..  Sci Transl Med,  (287): (287ra70).  [PMID:25972002]
9. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki.  (2006-03-24)  Human chromosome 11 DNA sequence and analysis including novel gene identification..  Nature,  440  ((7083)): (497-500).  [PMID:16554811]
10. Tedder, T F TF, Streuli, M M, Schlossman, S F SF and Saito, H H..  (1988)  Isolation and structure of a cDNA encoding the B1 (CD20) cell-surface antigen of human B lymphocytes..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2448768]
11. Einfeld, D A DA, Brown, J P JP, Valentine, M A MA, Clark, E A EA and Ledbetter, J A JA..  (1988)  Molecular cloning of the human B cell CD20 receptor predicts a hydrophobic protein with multiple transmembrane domains..  The EMBO journal,      [PMID:2456210]
12. Tedder, T F TF, Klejman, G G, Schlossman, S F SF and Saito, H H..  (1989)  Structure of the gene encoding the human B lymphocyte differentiation antigen CD20 (B1)..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:2466899]
13. Stamenkovic, I I and Seed, B B..  (1988)  Analysis of two cDNA clones encoding the B lymphocyte antigen CD20 (B1, Bp35), a type III integral membrane protein..  The Journal of experimental medicine,    (1):   [PMID:3260267]
14. Tedder, T F TF, Boyd, A W AW, Freedman, A S AS, Nadler, L M LM and Schlossman, S F SF..  (1985)  The B cell surface molecule B1 is functionally linked with B cell activation and differentiation..  Journal of immunology (Baltimore, Md. : 1950),      [PMID:3925015]
15. Bubien, J K JK, Zhou, L J LJ, Bell, P D PD, Frizzell, R A RA and Tedder, T F TF..  (1993)  Transfection of the CD20 cell surface molecule into ectopic cell types generates a Ca2+ conductance found constitutively in B lymphocytes..  The Journal of cell biology,      [PMID:7684739]
16. and Goldenberg, D M DM..  ()  The role of radiolabeled antibodies in the treatment of non-Hodgkin's lymphoma: the coming of age of radioimmunotherapy..  Critical reviews in oncology/hematology,      [PMID:11418316]
17. Chen, X X, Ji, Z L ZL and Chen, Y Z YZ..  (2002)  TTD: Therapeutic Target Database..  Nucleic acids research,    (1):   [PMID:11752352]
18. and Dillman, R O RO..  ()  Monoclonal antibody therapy for lymphoma: an update..  Cancer practice,      [PMID:11879282]
19. .  (2003)  Iodine-131 Tositumomab: (131)I-anti-B1 antibody, (131)I-tositumomab, anti-CD20 murine monoclonal antibody-I-131, B1, Bexxar, (131)I-anti-B1 antibody, iodine-131 tositumomab, iodine-131 anti-B1 antibody, tositumomab..  BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy,      [PMID:12899647]
20. Li, Haidong H, Ayer, Linda M LM, Lytton, Jonathan J and Deans, Julie P JP..  (2003)  Store-operated cation entry mediated by CD20 in membrane rafts..  The Journal of biological chemistry,    (24):   [PMID:12920111]
21. Du, Jiamu J and 8 more authors..  (2008)  Crystal structure of chimeric antibody C2H7 Fab in complex with a CD20 peptide..  Molecular immunology,      [PMID:18346788]
22. Dörner, Thomas T and Burmester, Gerd R GR..  (2008)  New approaches of B-cell-directed therapy: beyond rituximab..  Current opinion in rheumatology,      [PMID:18388516]
23. Polyak, Maria J MJ, Li, Haidong H, Shariat, Neda N and Deans, Julie P JP..  (2008)  CD20 homo-oligomers physically associate with the B cell antigen receptor. Dissociation upon receptor engagement and recruitment of phosphoproteins and calmodulin-binding proteins..  The Journal of biological chemistry,    (4):   [PMID:18474602]
24. Niederfellner, Gerhard G and 16 more authors..  (2011)  Epitope characterization and crystal structure of GA101 provide insights into the molecular basis for type I/II distinction of CD20 antibodies..  Blood,    (14):   [PMID:21444918]
25. Ivaldi, Corinne C and 6 more authors..  (2012)  Proteomic analysis of S-acylated proteins in human B cells reveals palmitoylation of the immune regulators CD20 and CD23..  PloS one,      [PMID:22615937]
26. Phuah, Jiayao J and 10 more authors..  (2016)  Effects of B Cell Depletion on Early Mycobacterium tuberculosis Infection in Cynomolgus Macaques..  Infection and immunity,      [PMID:26883591]
27. Hayashi, Kazumi and 6 more authors..  (2016)  Gemcitabine enhances rituximab-mediated complement-dependent cytotoxicity to B cell lymphoma by CD20 upregulation..  Cancer science,      [PMID:26920337]

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