The page color rapid gel preparation kit (12.5%) uses the formula in the form of pre mixed liquid, and only needs to add the improved coagulant when preparing the gel, which is convenient and fast. The upper glis color (red) gel, which is convenient for sampling. The gel prepared by this product can also be used in non denaturing PAGE gel electrophoresis experiments. This kit is equipped with an improved coagulant, which has no TEMED pungent odor, good stability and catalytic effect. It only takes about 20 minutes to prepare one or more gels. For easy access, the opened modified coagulant can be stored at 4 ℃ for at least 3 months. Glmaking quantity (125 gels): 125 pieces (0.75 mm glue); ＞ 90 pieces (1.00 mm); ＞ 60 pieces (1.50 mm)
Matters needing attention：
1. please centrifuge the product to the bottom of the tube immediately before use, and then conduct subsequent experiments. 2. the upper gel prepared by this product has no concentration effect on the sample, which is similar to the prefabricated gel. Compared with the traditional PAGE gel, the protein band separation effect is better, small molecular proteins (such as 10 kDa) can also be clearly separated, and the protein band is narrower and sharper. 3. the dosage of improved coagulant can be adjusted according to personal habits and experience. Adding more coagulant can accelerate the gel, and vice versa. Under the same conditions, the higher the temperature, the faster the gel speed. It is recommended to reduce the amount of coagulant appropriately when the temperature is too high. 4. please restore all reagents to room temperature before use, which can effectively avoid the generation of bubbles during the gel process. 5. the gel prepared by this product can be used for non denaturing PAGE gel electrophoresis. 6. for your safety and health, please wear experimental clothes and disposable gloves for operation
