Recombinant protein of human Pancreatic Lipase (AA 1-465)
Positive Control
WB: Mouse pancreas, A549 and C6 lysates. IHC: Rat kidney tissue.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Product Description
Rabbit anti Human Pancreatic Lipase/PTL Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Pancreatic Lipase/PTL Antibody (Ab120186) - Western Blot All lanes: Pancreatic Lipase/PTL Antibody (Ab120186) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 47 kDa Exposure time: 13.2 s
Pancreatic Lipase/PTL Antibody (Ab120186) - Western Blot All lanes: Pancreatic Lipase/PTL Antibody (Ab120186) at 1/1000 dilution Samples: Lysates at 10 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 47 kDa Exposure time: 60.0 s
Pancreatic Lipase/PTL Antibody (Ab120186) - IHC Immunohistochemical analysis of Pancreatic Lipase staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Pancreatic Lipase/PTL Antibody (Ab120186) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.