mAb (3H2-F8-B5); Mouse anti Human PDHA1 Antibody; WB, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab120952-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab120952-50μl | 50μl | In stock | $189.90 | |
| Ab120952-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab120952-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,249.90 |
mAb (3H2-F8-B5); Mouse anti Human PDHA1 Antibody; WB, ICC, IF; Unconjugated
| Product Name | PDHA1 Mouse mAb - Primary antibody, specific to PDHA1, Mouse IgG1 |
|---|---|
| Synonyms | ODPA_HUMAN antibody; PDH antibody; PDHA antibody; PDHA1 antibody; PDHCE1A antibody; PDHE1 A type Iantibody; PDHE1-A type I antibody; PHE1A antibody; Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody; Pyruvate dehydrogenase complex, E1 alpha polypeptide |
| Specifications & Purity | ExactAb™, Validated, 1.14 mg/mL |
| Host species | Mouse |
| Specificity | PDHA1 |
| Immunogen | Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli (AA 131-389). |
| Positive Control | WB: HeLa, HEK293, Hep G2, A549, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description | Mouse anti Human PDHA1 Antibody, Monoclonal (3H2-F8-B5), could be used for WB, ICC, IF and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 1.14 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
PDHA1 Mouse mAb (Ab120952) - Western Blot
All lanes: PDHA1 Mouse mAb (Ab120952) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 41 kDa
Exposure time: 20.0 s
PDHA1 Mouse mAb (Ab120952) - IF/ICC
Immunocytochemistry analysis of Pyruvate Dehydrogenase E1 alpha in HeLa using PDHA1 Mouse mAb (Ab120952).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0607376 | Certificate of Analysis | Jun 25, 2024 | Ab120952 |
| ZJ23F1202126 | Certificate of Analysis | Dec 20, 2023 | Ab120952 |
| ZJ23F1202125 | Certificate of Analysis | Dec 20, 2023 | Ab120952 |
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| 31. Ross, Mark T MT and 281 more authors.. (2005) The DNA sequence of the human X chromosome.. Nature, (17): [PMID:15772651] |
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| 33. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 34. Kato, Masato M and 6 more authors.. (2008) Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops.. Structure (London, England : 1993), (10): [PMID:19081061] |
| 35. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 36. de Ligt, Joep J and 16 more authors.. (2012) Diagnostic exome sequencing in persons with severe intellectual disability.. The New England journal of medicine, (15): [PMID:23033978] |
| 37. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 38. Parrini, Elena E and 18 more authors.. (2017) Diagnostic Targeted Resequencing in 349 Patients with Drug-Resistant Pediatric Epilepsies Identifies Causative Mutations in 30 Different Genes.. Human mutation, [PMID:27864847] |
