Peretinoin is an oral acyclic retinoid with a vitamin A-like structure that targets retinoid nuclear receptors such as retinoid X receptor (RXR) and retinoic acid receptor (RAR) . Peretinoin reduces the mRNA level of sphingosine kinase 1 (SPHK1) in vitro
Storage Temp
Protected from light,Store at -20°C,Argon charged
Shipped In
Ice chest + Ice pads
Product Description
Peretinoin is an oral acyclic retinoid with a vitamin A-like structure that targets retinoid nuclear receptors such as retinoid X receptor (RXR) and retinoic acid receptor (RAR). Peretinoin reduces the mRNA level of sphingosine kinase 1 (SPHK1) in vitro by downregulating a transcription factor, Sp1. Peretinoin prevents the progression of non-alcoholic steatohepatitis (NASH) and the development of hepatocellular carcinoma (HCC) through activating the autophagy pathway by increased Atg16L1 expression . Peretinoin inhibits HCV RNA amplification and virus release by altering lipid metabolism with a EC 50 of 9 μM .
In Vitro
Peretinoin (10-40?μM; 12-72?hours) exhibits suppressed SPHK1 expression after 24?h treatment, even at 10?μM and more prominent after 72 h peretinoin treatment. Peretinoin? (5?μM; 24?hours) up-regulates the expression of LC3B-II and increases autophagy flux in mouse primary hepatocytes. ? MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Autophagy AssayCell Line: Mouse primary hepatocytes (MPH) and the human HCC HepG2 cell line Concentration: 5 μM Incubation Time: 24 hours Result: Up-regulated the expression of LC3B-II and increased autophagy flux. Western Blot AnalysisCell Line: Human hepatoma (Huh-7) cells Concentration: 10, 20 and 40 μM Incubation Time: 12, 24, 48 and 72 hours Result: Exhibited suppressed SPHK1 expression after 24 h treatment, even at 10 μM.
