Phaseolus vulgaris lectin E (PHA-E)

Item Number

P140098

• Synonyms: Lectin from Phaseolus vulgaris (red kidney bean);Phaseolus vulgaris agglutinin; PHA
• Storage Temp: Store at -20°C
• Shipped In: Ice chest + Ice pads

Product Description

Phaseolus vulgaris lectin (PHA-E) is isolated from red kidney beans and purified by affinity chromatography and ion-exchange chromatography. It is a tetrameric protein with a molecular weight of 128 kDa. The lectin recognizes and binds specifically to terminal galactose, N-acetylglucosamine and mannose residues of complex glycans on mammalian glycoproteins. Because of its strong erythrocyte agglutinating activity, it is also known as Erythroagglutinin (PHA-E). PHA-E is not blood group specific. Less than 5 μg/ml agglutinates human erythrocytes of type O and agglutination can be inhibited by certain oligosaccharides. PHA-E lectin is supplied as a white lyophilized powder from a buffer containing from 50 mM NH4HCO3. The purity of the lectin in determined by SDS-PAGE, which generate one single band at 34 kDa.
● Ultrapure quality
● Sugar specificity: complex oligosaccharides
● Strong erythroagglutinin activity
● Lyophilized powder
Erythrocyte agglutination studies;Model system of how proteins recognize carbohydrates