| Product Description | Aladdin's Phosphatase Inhibitor Cocktail D (50X) can be used to inhibit phosphatase activity in protein extracts of animal cells or tissues, fungi, bacteria, and plants. It contains 2.5mM (-)-p-bromotetramisole oxalate, 500μM Cantharidin and 1μM Calyculin A in high-purity DMSO, with an inhibitory effect on alkaline and Ser/Thr phosphatases.Many endogenous proteases and phosphatases are commonly present in cell or tissue extracts, which easily leads to degradation or dephosphorylation of protein samples. Therefore, adding appropriate inhibitors is generally necessary for protein extraction.Different types of protein extracts contain different types of proteases. For example, there is high abundance of serine proteases in mammalian cell extracts, more serine proteases and metalloproteases inbacterial cell extract, and serine, cysteine and metalloproteases in mammalian tissue extracts. Plant extracts often contain large amounts of serine and cysteine proteases, and some contain aspartic proteases. Therefore, aladdin developed a variety of inhibitor cocktails for the protein extraction from different types of samples. Please refer to Table 1 in Appendix for details.This product can effectively inhibit alkaline phosphatase and serine/threonine phosphatase in animal tissue protein extracts. to maintain the phosphorylation state of proteins. Among its components, (-)-p-bromotetramisole oxalate is an irreversible inhibitor of alkaline phosphatase. Cantharidin is a reversible inhibitor of protein phosphatase 2A with IC50 of 40nM. Calyculin A is a reversible inhibitor of serine/threonine protein phosphatase 1 and protein phosphatase 2A. Please refer to Table 2 in Appendix for specific information of various inhibitors.This product is usually sufficient for preparing 100ml of lysis buffer.
Precautions： It is recommended to store this product in aliquot. This product should be added into solutions immediately before use.Phosphatase inhibitors contained in this product are harmful to human. Please take effective measures to avoid direct contact or inhalation.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Appendix：Table 1. Inhibitor cocktails from .Cat. No.Product NamePack SizeP1005Protease Inhibitor Cocktail for General Use, 100X1mlP1006Protease Inhibitor Cocktail for General Use, 100X5mlP1008Protease inhibitor cocktail for general use, MS-SAFE, 50X1mlP1009Protease inhibitor cocktail for general use, MS-SAFE, 50X5mlP1010Protease inhibitor cocktail for mammalian cell and tissue extracts, 100X1mlP1011Protease inhibitor cocktail for mammalian cell and tissue extracts, 100X5mlP1015Protease inhibitor cocktail for plant cell and tissue extracts, 100X1mlP1016Protease inhibitor cocktail for plant cell and tissue extracts, 100X5mlP1020Protease inhibitor cocktail for fungal and yeast extracts, 100X1mlP1021Protease inhibitor cocktail for fungal and yeast extracts, 100X5mlP1025Protease inhibitor cocktail for bacterial cell extracts, 100X1mlP1026Protease inhibitor cocktail for bacterial cell extracts, 100X5mlP1030Protease inhibitor cocktail for purification of His-tagged proteins, 100X1mlP1031Protease inhibitor cocktail for purification of His-tagged proteins, 100X5mlP1045Protease and phosphatase inhibitor cocktail for general use, 50X2ml eachP1046Protease and phosphatase inhibitor cocktail for general use, 50X10ml eachP1048Protease and phosphatase inhibitor cocktail for general use, MS-safe, 50X1ml eachP1049Protease and phosphatase inhibitor cocktail for general use, MS-safe, 50X5ml eachP1050Protease and phosphatase inhibitor cocktail for mammalian cell and tissue extracts, 50X2ml eachP1051Protease and phosphatase inhibitor cocktail for mammalian cell and tissue extracts, 50X10ml eachP1055Protease and phosphatase inhibitor cocktail for plant cell and tissue extracts, 50X2ml eachP1056Protease and phosphatase inhibitor cocktail for plant cell and tissue extracts, 50X10ml eachP1060Protease and phosphatase inhibitor cocktail for fungal and yeast extracts, 50X2ml eachP1061Protease and phosphatase inhibitor cocktail for fungal and yeast extracts, 50X10ml eachP1065Protease and phosphatase inhibitor cocktail for bacterial cell extracts, 50X2ml eachP1066Protease and phosphatase inhibitor cocktail for bacterial cell extracts, 50X10ml eachP1081Phosphatase inhibitor cocktail A, 50X2mlP1082Phosphatase inhibitor cocktail A, 50X10mlP1086Phosphatase inhibitor cocktail B ,50X2mlP1087Phosphatase inhibitor cocktail B ,50X10mlP1091Phosphatase inhibitor cocktail C ,50X2mlP1092Phosphatase inhibitor cocktail C ,50X10mlP1096Phosphatase inhibitor cocktail D ,50X2mlP1097Phosphatase inhibitor cocktail D ,50X10mlP1112Deacetylase Inhibitor Cocktail,100X1mlP1113Deacetylase Inhibitor Cocktail,100X5mlTable 2. Information of commonly used protease, phosphatase, and deacetylase inhibitors. NameMW Target EnzymeInhibition AEBSF239.5Serine proteaseirreversibleAprotinin6511.5Serine proteaseReversibleBestatin308.38AminopeptidaseReversibleE64357.4Cysteine proteaseirreversibleLeupeptin475.6Serine and Cysteine proteaseReversiblePepstatin A685.9Aspartic proteaseReversibleEDTA372.24MetalloproteinaseReversiblePhenanthroline198.22MetalloproteinaseReversiblePhosphoramidon587.5MetalloproteinaseReversibleSodium fluoride41.99Acid phosphataseReversibleSodium pyrophosphate221.9Serine/Threonine Phosphataseirreversibleβ-glycerophosphate306.11Serine/Threonine PhosphataseReversibleImidazole68.2Alkaline phosphataseReversibleSodium molybdate205.9Acid phosphataseirreversibleSodium tartrate 230.1Acid phosphataseReversibleSodium orthovanadate183.91Alkaline and Tyrosine PhosphatasesReversible(-)-p-Bromotetramisole373.22Alkaline phosphataseirreversibleCantharidin196.20Protein Phosphatase 2AReversibleMicrocystin-LR995.17Protein Phosphatase 1 and Protein Phosphatase 2AReversibleCalyculin A1009.17Protein Phosphatase 1 and Protein Phosphatase 2AReversibleTrichostatin A302.37class I/II HDACReversibleEX-527248.71SIRT1 over SIRT2 & SIRT3ReversibleNicotinamide122.12class III HDACReversibleSodium butyrate110.09class I/II HDACReversible
Instructions for Use： This product is a 50X concentrated stock solution, and should be added to lysis buffer at a ratio of 1:50 for use (e.g., add 20μl of phosphatase inhibitor cocktail to 1ml of lysis buffer). The lysis buffer containing phosphatase inhibitors should be freshly prepared. After preparation, protein extraction from cells or tissues should be performed immediately.
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