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Basic Description
| Storage Temp | Protected from light,Store at -20°C,Desiccated |
|---|---|
| Shipped In | Ice chest + Ice pads |
| Product Description | Product introduction： The thiol-reactive pH-sensitive pHrodo™ Green Maleimide dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo™ Green dramatically increases fluorescence as the pH of its surroundings become more acidic. • Use pH-sensitive pHrodo™ Green Maleimide to make pH-sensitive bioconjugates of your choice • Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps or quenchers • Multiplex with red fluorescent markers such as RFP, pHrodo™ Red, and many others The increase in fluorescence of pHrodo™ Green as pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it an ideal tool to study endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo™ Green makes it useful for multi-color experiments. pHrodo™ Green can be detected using most standard green fluorescent filter sets and has been validated for use on a variety of platforms including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo™ Green in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow. pHrodo™ Green Maleimide is a thiol-reactive dye that can be used to create pHrodo™ Green bioconjugates in aqueous buffer. The maleimide reacts with free sulphydryl groups produced by the reduction of cysteines in proteins or peptides. Maleimides are particularly useful for labeling antibodies as the dye will not attach to the antibody binding site. This reaction will result in a stable conjugate that can be used in live cell assays or stored for later use. pHrodo™ Green is also available in an amine-reactive form.In addition, pHrodo™ Red reactive dyes and ready-to-use conjugates are available as a color alternative with the same properties. For Research Use Only. Not for human or animal therapeutic or diagnostic use. Color:Green |
Product Properties
| Extinction coefficient | 75000 |
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| Reactive group | Thiol |
| Reactivity | Thiol |
Certificates
Certificate of Analysis(COA)
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Chemical and Physical Properties
| Solubility | DMSO(Dimethylsulfoxide) |
|---|
Related Documents
References
| 1. Surana NK, Buscher AZ, Hardy GG, Grass S, Kehl-Fie T, St Geme JW. () Translocator proteins in the two-partner secretion family have multiple domains.. J Biol Chem, [PMID:16648638] |
| 2. Smith PL, Yellen G. () Fast and slow voltage sensor movements in HERG potassium channels.. J Gen Physiol, [PMID:11865022] |
| 3. De Keyzer J, Van Der Does C, Driessen AJ. () Kinetic analysis of the translocation of fluorescent precursor proteins into Escherichia coli membrane vesicles.. J Biol Chem, [PMID:12226104] |
| 4. Buskiewicz I, Peske F, Wieden HJ, Gryczynski I, Rodnina MV, Wintermeyer W,. () Conformations of the signal recognition particle protein Ffh from Escherichia coli as determined by FRET.. J Mol Biol, [PMID:16005894] |
| 5. Gambhir A, Hangyás-Mihályné G, Zaitseva I, Cafiso DS, Wang J, Murray D, Pentyala SN, Smith SO, McLaughlin S. () Electrostatic sequestration of PIP2 on phospholipid membranes by basic/aromatic regions of proteins.. Biophys J, [PMID:15041659] |
| 6. Miller RA, Presley AD, Francis MB. () Self-assembling light-harvesting systems from synthetically modified tobacco mosaic virus coat proteins.. J Am Chem Soc, [PMID:17319656] |
| 7. Kuiper JM, Pluta R, Huibers WH, Fusetti F, Geertsma ER, Poolman B,. () A method for site-specific labeling of multiple protein thiols.. Protein Sci, [PMID:19388048] |
| 8. La Clair JJ, Foley TL, Schegg TR, Regan CM, Burkart MD. () Manipulation of carrier proteins in antibiotic biosynthesis.. Chem Biol, [PMID:15123281] |
| 9. Buskiewicz I, Deuerling E, Gu SQ, Jöckel J, Rodnina MV, Bukau B, Wintermeyer W,. () Trigger factor binds to ribosome-signal-recognition particle (SRP) complexes and is excluded by binding of the SRP receptor.. Proc Natl Acad Sci U S A, [PMID:15148364] |