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PKM Antibody - Primary antibody, specific to PKM, Rabbit IgG

  • Azide Free
  • Carrier Free
  • ExactAb™
  • High performance
  • Validated
  • Lot by Lot
Features and benefits
  • Short Overview:

    pAb; Rabbit anti Human PKM Antibody; WB, IHC, ICC, IF; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • ICC/IF
  • IHC
  • WB
Item Number
Ab122143
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab122143-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$56.90
Ab122143-20μl
20μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$754.90
Ab122143-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$169.90
Ab122143-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$279.90
Ab122143-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,299.90

pAb; Rabbit anti Human PKM Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Accession#:P14618 Gene ID:5315 PKM PKM2

Basic Description

Product NamePKM Antibody - Primary antibody, specific to PKM, Rabbit IgG
SynonymsCTHBP antibody; Cytosolic thyroid hormone-binding protein antibody; KPYM_HUMAN antibody; OIP-3 antibody; Opa-interacting protein 3 antibody; p58 antibody; pkm antibody; PKM1 antibody; PKM2 antibody; Pyruvate kinase 2/3 antibody; Pyruvate kinase muscle iso
Specifications & PurityExactAb™, Validated, Carrier Free, Azide Free, High performance, Lot by Lot
Host speciesRabbit
SpecificityPKM
ImmunogenRecombinant PKM2 expressed in E.coli (AA 141-248)
Positive ControlWB: K562, MCF7, HeLa, HepG2, A431, THP-1, Raji, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue; Human skin cancer tissue, Human prostate tissue, Human prostate cancer tissue, Human colorectal cancer tissue, Human liver cancer tissue. ICC/IF
ConjugationUnconjugated
GradeAzide Free, Carrier Free, ExactAb™, High performance, Validated
Product Description

Rabbit anti Human PKM Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on.
Application
WB: 0.01-2 µg/mL
IHC: 5-20 µg/mL
ICC/IF: 5-20 μg/mL
Protein Function
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified and Protein A purified
FormLiquid
ConcentrationLot by Lot
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

PKM Antibody (Ab122143) - Western Blot
All lanes: PKM Antibody (Ab122143) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 1.6 s

PKM Antibody (Ab122143) - IF/ICC
FITC staining on IF
Samples: Human MCF7 cells
Primary Ab: 20 ug/mL PKM Antibody (Ab122143)
Second Ab: 1 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Skin cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Prostate Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Prostate cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Associated Targets

PKM Tchem Pyruvate kinase PKM 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

ICC/IF

5-20 µg/mL

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1001237Certificate of AnalysisOct 10, 2023 Ab122143
ZJ23F1001236Certificate of AnalysisOct 10, 2023 Ab122143

Related Documents

References

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