This plant protein extraction kit can extract soluble plant proteins from fresh, frozen, or dried plant tissues. Suitable for protein extraction from various plants and different parts of plants (such as roots, stems, leaves, flowers, seeds, etc.), with high extraction efficiency, high protein yield, high activity, and fast speed. The extracted protein can be directly subjected to protein electrophoresis analysis, immunoprecipitation, Western Blot, protein activity determination, and protein purification experiments. The concentration of the extracted protein can be determined using the BCA protein quantification kit .
P665757
Component
100 T
Storage
P665757A
Plant Protein Extraction Reagent
100 mL
RT
P665757B
Protease Inhibitor Cocktail (100×)
1 mL
-20℃. Avoid freeze/thaw cycle.
Precautions： 1. This product contains 1mM EDTA. 2. To prevent protein degradation, all operations should be carried out on ice as much as possible. 3. After extracting protein using this product, the BCA method can be used for protein quantification. 4. To achieve the best experimental results, please adjust the optimal usage amount according to the experiment.
Operation steps: 1. Please remove the required Plant Protein Extraction Agent for pre cooling before protein extraction. 2. Weigh the weight of the experimental plant tissue. Add 5 ml of Plant Protein Extraction Agent to 1 g of tissue (add Protein Inhibitor Cocktail in a 1:99 ratio before protein extraction). Attention: 1) Before homogenization, cut large pieces of plant tissue into small pieces and homogenize them with a mechanical homogenizer for 10 seconds, with an interval of 10 seconds. Repeat the process three times and select the appropriate homogenization method according to the different tissue samples. 2) The amount of lysate used is adjusted according to different parts of the plant. If concentrated protein extracts are needed, the amount of Plant Protein Extraction Agent used can be appropriately reduced. 3. After homogenization, incubate on ice for 20-30 minutes. 4.4 ℃ 13400 × g, centrifuge for 20 minutes. 5. Collect soluble proteins from the supernatant for further purification or downstream analysis.
