PMSF - 10mM in DMSO, high purity , CAS No.329-98-6(DMSO)

  • 10mM in DMSO
Item Number
P408676
Grouped product items
SKUSizeAvailabilityPrice Qty
P408676-1ml
1ml
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$161.90

Serine Protease Inhibitors

Basic Description

Specifications & Purity10mM in DMSO
Biochemical and Physiological MechanismsPMSF (Phenylmethylsulfonyl Fluoride, Benzylsulfonyl fluoride) is an irreversible serine/cysteine protease inhibitor.
Storage TempStore at -80°C
Shipped InIce chest + Ice pads
Product Description

Information

PMSF PMSF (Phenylmethylsulfonyl Fluoride, Benzylsulfonyl fluoride) is an irreversible serine/cysteine protease inhibitor.
In vitro

Although human trypsin is less susceptible to inhibition by PMSF, PMSF rapidly inactivates purified chymotrypsin from human pancreas. PMSF also rapidly inhibits acetylcholinesterase from human red cells. As an inhibitor of phosphatidylinositol-specific phospholipase C, PMSF treatment at 2 mM almost completely inhibits carbachol-stimulated inositol incorporation into phosphatidylinositol (PI) of longitudinal smooth muscle of guinea pig ileum, while it has no effect on potassium-stimulated inositol incorporation. In contrast to its specific inhibition of carbachol-stimulated phosphoinositide turnover, PMSF produces a transient inhibition of contraction by both carbachol and potassium. PMSF has been shown to inhibit the addition of ethanolamine phosphate to glycosylphosphatidylinositol (GPI) intermediates in Trypanosoma brucei. PMSF also inhibits the acylation of the inositol residue of GPI intermediates in bloodstream form T. brucei. PMSF inhibits ethanolamine phosphate addition and inositol acylation for procyclic forms of T. brucei but not for mammalian HeLa cells. PMSF is the more reactive inactivator of mouse acetylcholinesterase (AChE), as the 8-fold higher BSF concentration is necessary to achieve even a 6-fold slower inactivation than that using PMSF.

In vivo

Intraperitoneal injection of PMSF produces dose-dependent analgesia in Sprague-Dawley rats. PMSF significantly enhances the analgesic effect of beta-endorphin (END) in rats. Mice receiving i.p. injections of PMSF exhibit cannabinoid effects that include antinociception, hypothermia and immobility with ED50 of 86 mg/kg, 224 mg/kg and 206 mg/kg, respectively. Pretreatment with an inactive dose of PMSF (30 mg/kg) enhances the effects of anandamide on tail-flick response (antinociception), spontaneous activity and mobility by 5-, 10- and 8-fold, respectively. Administration of PMSF 12 hours prior to PSP causes complete protection in organophosphorus ester-induced delayed neuropathy (OPIDN) in hens, but PMSF administered 4 hours after PSP potentiates its neurotoxic effects. Pretreatment with PMSF (30 mg/kg, i.p.) prior to an injection of 1 or 10 mg/kg 3H-anandamide results 5 minutes later in enhanced brain levels of anandamide compared to those obtained with 3H-anandamide plus vehicle injection. Pretreatment with PMSF inhibits tri-ortho-cresyl phosphate (TOCP)-induced neurofilament (NF) degradation, and protects hens against the development of organophosphate-induced delayed neuropathy (OPIDN). Administration of PMSF enhances the characteristic cannabimimetic effects of Δ(9)-tetrahydrocannabinol (THC) or anandamide (AEA) in ICR mice, by inhibiting the enzyme fatty acid amide hydrolase.
Cell Data

cell lines:

Concentrations:

Incubation Time:

Powder Purity:≥98%

Names and Identifiers

Canonical SMILES F[S](=O)(=O)CC1=CC=CC=C1
UN Number 2928
Packing Group II
Molecular Weight 174.19

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Chemical and Physical Properties

SolubilitySolubility (25°C) In vitro DMSO: 100 mg/mL (124.53 mM);    

Safety and Hazards(GHS)

Pictogram(s) GHS06,   GHS05
Signal Danger
Hazard Statements

H301:Toxic if swallowed

H314:Causes severe skin burns and eye damage

Precautionary Statements

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P301+P310:IF SWALLOWED: Immediately call a POISON CENTER/doctor/...

P310:Immediately call a POISON CENTER or doctor/physician.

P405:Store locked up.

P501:Dispose of contents/container to ...

P264:Wash hands [and …] thoroughly after handling.

P260:Do not breathe dust/fume/gas/mist/vapors/spray.

P303+P361+P353:IF ON SKIN (or hair): Take off Immediately all contaminated clothing. Rinse SKIN with water [or shower].

P270:Do not eat, drink or smoke when using this product.

P301+P330+P331:IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.

P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing.

P363:Wash contaminated clothing before reuse.

P330:Rinse mouth.

Class 6.1 (8)

Related Documents

Citations of This Product

1. Jiake Yu, Shuying Wang, Jie Yang, Wuxinrui Huang, Beikang Tang, Weijun Peng, Jing Tian.  (2023)  Exploring the mechanisms of action of Zengye decoction (ZYD) against Sjogren’s syndrome (SS) using network pharmacology and animal experiment.  PHARMACEUTICAL BIOLOGY,  61  (1): (1286-1297).  [PMID:37606264]
2. Xiuwen Zheng, Yuxin Zhang, Jian Xu, Xiangyi Li, Huan Wen, Xuguang Zhang, Aixia Liu, Junqing Zhang.  (2023)  Exploring the Underlying Mechanism of Alpinia officinarum Hance Ameliorating Diabetic Gastroparesis through Combining Network Pharmacology, Molecular Docking, and in Vivo Experimental Verification.  Frontiers in Bioscience-Landmark,  28  (8): (164).  [PMID:37664921]
3. Yujun Zhao, Xiaotie Wang, Keke Nie.  (2023)  IRF1 promotes the chondrogenesis of human adipose-derived stem cells through regulating HILPDA.  TISSUE & CELL,  82  (102046).  [PMID:36933274]
4. Zhe Chen, Yunxia Wang.  (2023)  A label- and enzyme-free fluorescence assay based on thioflavin T–induced G-quadruplexes for the detection of telomerase activity.  JOURNAL OF CHEMICAL RESEARCH,      [PMID:]
5. Yinxuan Lin, Jiao Yu, Meng Wang, Lingling Wu.  (2023)  Toxicity of single and combined 4-epianhydrotetracycline and cadmium at environmentally relevant concentrations on the zebrafish embryos (Danio rerio).  ENVIRONMENTAL POLLUTION,  316  (120543).  [PMID:36341832]
6. Huang Yang, Yuan Ding, Zongrui Tong, Xiaohui Qian, Hao Xu, Fenghao Lin, Guoping Sheng, Liangjie Hong, Weilin Wang, Zhengwei Mao.  (2022)  pH-responsive hybrid platelet membrane-coated nanobomb with deep tumor penetration ability and enhanced cancer thermal/chemodynamic therapy.  Theranostics,  12  (9): ( 4250–4268).  [PMID:35673566]
7. Weimin Lin, Xianyu Wen, Xuexin Li, Lei Chen, Wei Wei, Lifan Zhang, Jie Chen.  (2022)  MiR-144 regulates adipogenesis by mediating formation of C/EBPα-FOXO1 protein complex.  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,  612  (126).  [PMID:35525196]
8. Shi-Hao Mao, Dan-Dan Feng, Xi Wang, Yi-Hui Zhi, Shu Lei, Xi Xing, Rong-Lin Jiang, Jian-Nong Wu.  (2021)  Magnolol protects against acute gastrointestinal injury in sepsis by down-regulating regulated on activation, normal T-cell expressed and secreted.  World Journal of Clinical Cases,  (34): ( 10451–10463).  [PMID:35004977]
9. Liang Li,Ying Ma.  (2013-03-07)  Effect of fatty acids on the β-oxidation system and thioesterase of Lactococcus lactis subspecies lactis..  Journal of dairy science,  96  ((4)): (2003-2010).  [PMID:23462166]
10. Ting Tai,Huan Zhou,Ting Zhu,Yu-Meng Jia,Jin-Zi Ji,Yi-Fei Li,Qiong-Yu Mi,Hong-Guang Xie.  (2019-12-24)  Development and validation of a UPLC-MS/MS method for simultaneous determination of vicagrel and its major metabolites in rat or human plasma: An optimized novel strategy for the stabilization of vicagrel..  Journal of pharmaceutical and biomedical analysis,  179  (112955-112955).  [PMID:31866139]

References

1. Jiake Yu, Shuying Wang, Jie Yang, Wuxinrui Huang, Beikang Tang, Weijun Peng, Jing Tian.  (2023)  Exploring the mechanisms of action of Zengye decoction (ZYD) against Sjogren’s syndrome (SS) using network pharmacology and animal experiment.  PHARMACEUTICAL BIOLOGY,  61  (1): (1286-1297).  [PMID:37606264]
2. Xiuwen Zheng, Yuxin Zhang, Jian Xu, Xiangyi Li, Huan Wen, Xuguang Zhang, Aixia Liu, Junqing Zhang.  (2023)  Exploring the Underlying Mechanism of Alpinia officinarum Hance Ameliorating Diabetic Gastroparesis through Combining Network Pharmacology, Molecular Docking, and in Vivo Experimental Verification.  Frontiers in Bioscience-Landmark,  28  (8): (164).  [PMID:37664921]
3. Yujun Zhao, Xiaotie Wang, Keke Nie.  (2023)  IRF1 promotes the chondrogenesis of human adipose-derived stem cells through regulating HILPDA.  TISSUE & CELL,  82  (102046).  [PMID:36933274]
4. Zhe Chen, Yunxia Wang.  (2023)  A label- and enzyme-free fluorescence assay based on thioflavin T–induced G-quadruplexes for the detection of telomerase activity.  JOURNAL OF CHEMICAL RESEARCH,      [PMID:]
5. Yinxuan Lin, Jiao Yu, Meng Wang, Lingling Wu.  (2023)  Toxicity of single and combined 4-epianhydrotetracycline and cadmium at environmentally relevant concentrations on the zebrafish embryos (Danio rerio).  ENVIRONMENTAL POLLUTION,  316  (120543).  [PMID:36341832]
6. Huang Yang, Yuan Ding, Zongrui Tong, Xiaohui Qian, Hao Xu, Fenghao Lin, Guoping Sheng, Liangjie Hong, Weilin Wang, Zhengwei Mao.  (2022)  pH-responsive hybrid platelet membrane-coated nanobomb with deep tumor penetration ability and enhanced cancer thermal/chemodynamic therapy.  Theranostics,  12  (9): ( 4250–4268).  [PMID:35673566]
7. Weimin Lin, Xianyu Wen, Xuexin Li, Lei Chen, Wei Wei, Lifan Zhang, Jie Chen.  (2022)  MiR-144 regulates adipogenesis by mediating formation of C/EBPα-FOXO1 protein complex.  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,  612  (126).  [PMID:35525196]
8. Shi-Hao Mao, Dan-Dan Feng, Xi Wang, Yi-Hui Zhi, Shu Lei, Xi Xing, Rong-Lin Jiang, Jian-Nong Wu.  (2021)  Magnolol protects against acute gastrointestinal injury in sepsis by down-regulating regulated on activation, normal T-cell expressed and secreted.  World Journal of Clinical Cases,  (34): ( 10451–10463).  [PMID:35004977]
9. Liang Li,Ying Ma.  (2013-03-07)  Effect of fatty acids on the β-oxidation system and thioesterase of Lactococcus lactis subspecies lactis..  Journal of dairy science,  96  ((4)): (2003-2010).  [PMID:23462166]
10. Ting Tai,Huan Zhou,Ting Zhu,Yu-Meng Jia,Jin-Zi Ji,Yi-Fei Li,Qiong-Yu Mi,Hong-Guang Xie.  (2019-12-24)  Development and validation of a UPLC-MS/MS method for simultaneous determination of vicagrel and its major metabolites in rat or human plasma: An optimized novel strategy for the stabilization of vicagrel..  Journal of pharmaceutical and biomedical analysis,  179  (112955-112955).  [PMID:31866139]

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