Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.
Storage Temp
Store at 2-8°C
Shipped In
Wet ice
Grade
BioReagent, Proteomics grade
Product Description
Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.
Application:
PNGase F from Elizabethkingia meningoseptica has been used:
for de-N-glycosylation of Zika20virus E protein
to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells
to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)
Suitable for both proteomics and glycobiology use; compatible with MALDI-TOF MS analysis
