γ-irradiated, BioReagent, for cell culture, mol wt 70,000-150,000, lyophilized powder
Biochemical and Physiological Mechanisms
Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After
Storage Temp
Store at -20°C
Shipped In
Ice chest + Ice pads
Grade
BioReagent, for cell culture, γ-irradiated
Product Description
Poly-D-lysine hydrobromide has been used:
as a coating for plates used in culturing rat glial precursor cells (RGPCS).
in coating flasks and plates in culturing tanycytes
in preparing the surface of coverslips for facilitating cell attachment
Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.
This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.
