Poly-L-lysine hydrobromide

Item Number

P755717

• Specifications & Purity: γ-irradiated, BioReagent, for cell culture, mol wt 30,000-70,000, lyophilized powder
• Biochemical and Physiological Mechanisms: Poly-L-lysine is employed as a coating substance to facilitate improved cell attachment to culture surfaces. Research indicates that plates coated with poly-L-lysine (PLL) exhibit an accelerated cell growth rate. When mesenchymal stem cells (MSCs) are cul
• Storage Temp: Store at -20°C
• Shipped In: Ice chest + Ice pads
• Grade: BioReagent, for cell culture, γ-irradiated

Product Description

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties. Poly-L-lysine hydrobromide is a nonspecific attachment factor in promoting cell adhesion to the substratum.

Application:

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm². Optimal conditions for attachment must be determined for each cell line and application.
Poly-L-lysine hydrobromide has been used to adhere the dissociated cells of cortical tissues into the culture plates. It has also been used to treat slides, to fix samples on it for immunohistochemical analysis.