Poly-L-lysine hydrobromide

Item Number

P755733

• Specifications & Purity: γ-irradiated, BioReagent, for cell culture, mol wt 70,000-150,000, lyophilized powder
• Biochemical and Physiological Mechanisms: Poly-L-lysine serves as a pre-coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on P
• Storage Temp: Store at -20°C
• Shipped In: Ice chest + Ice pads
• Grade: BioReagent, for cell culture, γ-irradiated

Product Description

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Application:

Poly-L-lysine hydrobromide has been used to precoat glass bottom dishes used for in vitro cell culture.

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm². Optimal conditions for attachment must be determined for each cell line and application.