50 % (w/v), average mol wt 1,450, sterile-filtered, BioReagent, suitable for hybridoma
Storage Temp
Store at 2-8°C,Argon charged
Shipped In
Wet ice
Product Description
Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.
Application
Polyethylene glycol solution has been used in the hybridization of the following:
1, irradiated camel fibroblast cells with A23 hamster cells
2, mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
3, rabbit splenocytes and cell line 240E-1
Recommended for use in a normal fusion protocol requiring 50% PEG.PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.
Other Notes
Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.
Reconstitution
Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.
Names and Identifiers
Canonical SMILES
C(CO)O
WGK Germany
1
Certificates
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1.Pengfei Han, Yuewu Zhu, Jin Liu. (2015) An all-solid-state lithium ion battery electrolyte membrane fabricated by hot-pressing method. Journal of Power Sources, 284 (284):(459-465). [PMID:]
2.J Huang, Z Chen, C Wen, T Ling, Z Chen. (2022) Thermally assisted 3D printing of bio-polymer with high solute loading with improved mechanical properties. Additive Manufacturing, (59A):(103088). [PMID:]
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H2419548
