PPP2R4 Antibody - Primary antibody, specific to PPP2R4, Rabbit IgG
Synonyms
subunit B'' antibody; KIAA0044antibody; MGC2184antibody; Phosphotyrosyl phosphatase activator antibody; PP2A antibody; PP2A phosphatase activatorantibody; PP2A subunit B' antibody; PP2A subunit B' isoform PR53 antibody; PP2A subunit B' PR53 isoformantibod
Specifications & Purity
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species
Rabbit
Specificity
PPP2R4
Immunogen
A synthetic peptide derived from human PTPA (AA 347-358)
Positive Control
WB: Hep G2, HeLa, NIH/3T3 and C6 cell lysates. IHC: Human thyroid cancer tissue.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Product Description
Rabbit anti Human PPP2R4 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine-protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+) (By similarity). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activty of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
PPP2R4 Antibody (Ab122864) - Western Blot All lanes: PPP2R4 Antibody (Ab122864) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 41 kDa Observed band size: 43 kDa Exposure time: 4.1 s
PPP2R4 Antibody (Ab122864) - Western Blot All lanes: PPP2R4 Antibody (Ab122864) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 41 kDa Observed band size: 36, 43 kDa Exposure time: 1.0 s
PPP2R4 Antibody (Ab122864) - IHC Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using PPP2R4 Antibody (Ab122864). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.