pAb; Rabbit anti Human Profilin 1 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab123181-10μl | 10μl | In stock | $66.90 | |
| Ab123181-20μl | 20μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $99.90 | |
| Ab123181-50μl | 50μl | In stock | $189.90 | |
| Ab123181-100μl | 100μl | In stock | $289.90 | |
| Ab123181-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,449.90 |
pAb; Rabbit anti Human Profilin 1 Antibody; WB; Unconjugated
| Product Name | Profilin 1 Antibody - Primary antibody, specific to Profilin 1, Rabbit IgG |
|---|---|
| Synonyms | Actin binding proteinantibody; ALS18 antibody; Epididymis tissue protein Li 184a antibody; OTTHUMP00000125244 antibody; PFN 1 antibody; Pfn antibody; PFN1 antibody; PROF1_HUMAN antibody; Profilin I antibody; Profilin-1 antibody; Profilin1 antibody; Profil |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, High performance, Lot by Lot |
| Host species | Rabbit |
| Specificity | Profilin 1 |
| Immunogen | Recombinant PFN1 expressed in E.coli (AA 1-139) . |
| Positive Control | WB: HeLa, Jurkat, HEK293, HUVEC, Hep G2, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Rabbit anti Human Profilin 1 Antibody, Polyclonal, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified + Protein A purified |
| Form | Liquid |
| Concentration | Lot by Lot |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Profilin 1 Antibody (Ab123181) - Western Blot
All lanes: Profilin 1 Antibody (Ab123181) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 10.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB |
0.01-2 µg/mL
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303645 | Certificate of Analysis | Mar 22, 2024 | Ab123181 |
| ZJ24F0303644 | Certificate of Analysis | Mar 22, 2024 | Ab123181 |
| ZJ24F0303643 | Certificate of Analysis | Mar 22, 2024 | Ab123181 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Ampe, C C, Markey, F F, Lindberg, U U and Vandekerckhove, J J.. (1988) The primary structure of human platelet profilin: reinvestigation of the calf spleen profilin sequence.. FEBS letters, (8): [PMID:3342873] |
| 4. Kwiatkowski, D J DJ and Bruns, G A GA.. (1988) Human profilin. Molecular cloning, sequence comparison, and chromosomal analysis.. The Journal of biological chemistry, (25): [PMID:3356709] |
| 5. Gieselmann, R R, Kwiatkowski, D J DJ, Janmey, P A PA and Witke, W W.. (1995) Distinct biochemical characteristics of the two human profilin isoforms.. European journal of biochemistry, (1): [PMID:7758455] |
| 6. Metzler, W J WJ and 6 more authors.. (1993) Characterization of the three-dimensional solution structure of human profilin: 1H, 13C, and 15N NMR assignments and global folding pattern.. Biochemistry, (21): [PMID:8268157] |
| 7. Mahoney, N M NM, Janmey, P A PA and Almo, S C SC.. (1997) Structure of the profilin-poly-L-proline complex involved in morphogenesis and cytoskeletal regulation.. Nature structural biology, [PMID:9360613] |
| 8. Mahoney, N M NM, Rozwarski, D A DA, Fedorov, E E, Fedorov, A A AA and Almo, S C SC.. (1999) Profilin binds proline-rich ligands in two distinct amide backbone orientations.. Nature structural biology, [PMID:10404225] |
| 9. Stüven, Theis T, Hartmann, Enno E and Görlich, Dirk D.. (2003) Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes.. The EMBO journal, (3): [PMID:14592989] |
| 10. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 11. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455] |
| 12. Ferron, François F, Rebowski, Grzegorz G, Lee, Sung Haeng SH and Dominguez, Roberto R.. (2007) Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena/VASP.. The EMBO journal, (31): [PMID:17914456] |
| 13. Shao, Jieya J, Welch, William J WJ, Diprospero, Nicholas A NA and Diamond, Marc I MI.. (2008) Phosphorylation of profilin by ROCK1 regulates polyglutamine aggregation.. Molecular and cellular biology, [PMID:18573880] |
| 14. Baek, Kyuwon K and 5 more authors.. (2008) Modulation of actin structure and function by phosphorylation of Tyr-53 and profilin binding.. Proceedings of the National Academy of Sciences of the United States of America, (19): [PMID:18689676] |
| 15. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 16. Li, JianYuan J and 12 more authors.. (2010) Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins.. Molecular & cellular proteomics : MCP, [PMID:20736409] |
| 17. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 18. Wu, Chi-Hong CH and 37 more authors.. (2012) Mutations in the profilin 1 gene cause familial amyotrophic lateral sclerosis.. Nature, (23): [PMID:22801503] |
| 19. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912] |
