mAb (AB02/3H12); Mouse anti Human Profilin 1; WB, Flow, ELISA; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab183903-10μl (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab183903-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $189.90 | |
| Ab183903-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab183903-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
mAb (AB02/3H12); Mouse anti Human Profilin 1; WB, Flow, ELISA; Unconjugated
| Product Name | Profilin 1 Mouse mAb |
|---|---|
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE), 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androg |
| Host species | Mouse |
| Specificity | Profilin 1 |
| Immunogen | Recombinant protein corresponding to Human Profilin 1 |
| Positive Control | WB: HeLa, HepG2, HT-29, K562, HEK293, A431 and Jurkat whole cell lysates Flow: HeLa cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Mouse anti Human Profilin 1 Antibody, clone AB02/3H12, could be used for WB, Flow, ELISA and so on.
|
| Isotype | Mouse IgG2b |
|---|---|
| Light Chain Type | Lambda |
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
Profilin 1 Mouse mAb (Ab183903) - Western Blot
All lanes: Profilin 1 Mouse Antibody (Ab183903) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 16 kDa
Exposure time: 54.0 s
Profilin 1 Mouse mAb (Ab183903) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with Profilin 1 Mouse mAb (Ab183903) (Red) at 2.5 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG2b was used as an isotype control (Blue).
Profilin 1 Mouse mAb (Ab183903) - Western Blot
All lanes: Profilin 1 Mouse Antibody (Ab183903) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 15.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| Flow | 1/200 - 1/50,000 |
| WB | 1/1,000 - 1/100,000; Predicted molecular weight: 15.0 KDa |
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