Determine the necessary mass, volume, or concentration for preparing a solution.
Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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SKU | Size | Availability | Price | Qty |
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P266304-5mg | 5mg | In stock | $19.90 | |
P266304-25mg | 25mg | In stock | $56.90 | |
P266304-100mg | 100mg | In stock | $125.90 |
Synonyms | propidium iodide|25535-16-4|Propidium diiodide|3,8-Diamino-5-(3-(diethyl(methyl)ammonio)propyl)-6-phenylphenanthridin-5-ium iodide|Propidiumiodide|Propidium Iodine|Propidium (Iodide)|CHEBI:51240|3,8-Diamino-5-(3-(diethylmethylammonio)propyl)-6-phenylphena |
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Specifications & Purity | ≥98%(HPLC) |
Storage Temp | Protected from light,Store at -20°C,Desiccated |
Shipped In | Ice chest + Ice pads |
Product Description | PI is a nuclear staining reagent that can stain DNA. Like EB, PI intercalates into double-stranded DNA and releases red fluorescence. It is often used for the detection of apoptosis or necrosis, and is often used for flow cytometry analysis. Although PI cannot pass through living cell membranes, it can pass through damaged cell membranes to stain nuclei. PI is often used with Calcein, AM, Hoechst 33258 or Hoechst 33342, etc. It can stain live and dead cells at the same time. The excitation and emission wavelengths of the PI-DNA complex are 535nm and 615nm, respectively. Dyeing process (1) Prepare 10-50μM PI solution with PBS or appropriate buffer. (2) Add 1/10 of the volume of the medium to the cell culture medium (1/10 concentration of PI buffer can also be used to replace the medium). (3) Incubate the cells at 37°C for 10-20 minutes. (4) Wash the cells twice with PBS or a suitable buffer. (5) Observe the cells with a fluorescence microscope with a 535nm excitation wavelength and a 615nm emission wavelength filter. Storage conditions: Store at -20°C dry and dark, valid for one year. MSDS shows that propidium iodide PI is the same as EB mutagenic to germ cells and is a carcinogen. Please take strict care to protect it! Propidium iodide PI is a Germ cell mutagenicity germ cell mutant; In vitro tests showed mutagenic effects! |
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IUPAC Name | 3-(3,8-diamino-6-phenylphenanthridin-5-ium-5-yl)propyl-diethyl-methylazanium;diiodide |
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INCHI | InChI=1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1 |
InChi Key | XJMOSONTPMZWPB-UHFFFAOYSA-M |
Canonical SMILES | CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-] |
Isomeric SMILES | CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-] |
WGK Germany | 3 |
RTECS | SF7949600 |
PubChem CID | 104981 |
Molecular Weight | 668.39 |
Beilstein | 3843838 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
Lot Number | Certificate Type | Date | Item |
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K2317040 | Certificate of Analysis | Sep 04, 2024 | P266304 |
H2405417 | Certificate of Analysis | Jul 31, 2024 | P266304 |
H2405419 | Certificate of Analysis | Jul 31, 2024 | P266304 |
H2405421 | Certificate of Analysis | Jul 31, 2024 | P266304 |
E2416283 | Certificate of Analysis | May 13, 2024 | P266304 |
E2416299 | Certificate of Analysis | May 13, 2024 | P266304 |
E2416301 | Certificate of Analysis | May 13, 2024 | P266304 |
A2404015 | Certificate of Analysis | Jan 10, 2024 | P266304 |
A2404014 | Certificate of Analysis | Jan 09, 2024 | P266304 |
L2325053 | Certificate of Analysis | Jan 03, 2024 | P266304 |
G2327037 | Certificate of Analysis | Jul 13, 2023 | P266304 |
J2214030 | Certificate of Analysis | Jul 13, 2023 | P266304 |
J2214031 | Certificate of Analysis | Jul 13, 2023 | P266304 |
J2214036 | Certificate of Analysis | Jul 13, 2023 | P266304 |
J2310198 | Certificate of Analysis | Jul 13, 2023 | P266304 |
Sensitivity | Light sensitive;Moisture sensitive |
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Melt Point(°C) | 220-225°C |
Pictogram(s) | GHS08, GHS07 |
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Signal | Warning |
Hazard Statements | H315:Causes skin irritation H319:Causes serious eye irritation H335:May cause respiratory irritation H341:Suspected of causing genetic defects |
Precautionary Statements | P261:Avoid breathing dust/fume/gas/mist/vapors/spray. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of water. P321:Specific treatment (see ... on this label). P405:Store locked up. P501:Dispose of contents/container to ... P264:Wash hands [and …] thoroughly after handling. P281:Use personal protective equipment as required. P271:Use only outdoors or in a well-ventilated area. P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing. P403+P233:Store in a well-ventilated place. Keep container tightly closed. P362+P364:Take off contaminated clothing and wash it before reuse. P203:Obtain, read and follow all safety instructions before use. P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes. P318:if exposed or concerned, get medical advice. P337+P317:If eye irritation persists: Get medical help. P332+P317:If skin irritation occurs: Get medical help. P319:Get medical help if you feel unwell. |
WGK Germany | 3 |
RTECS | SF7949600 |