Basic Description

Synonyms	Nitrophenyl)methyl (4R,5S,6S)-6-[(1R)-1-hydroxyethyl]-4-methyl-7-oxo-3,4-diphenoxy-5-phosphonooxy-1-azabicyclo[3.2.0]hept-2-ene-2-carboxylate \| (4-nitrophenyl)methyl (4R,5S,6S)-6-[(1R)-1-hydroxyethyl]-4-methyl-7-oxo-3,4-diphenoxy-5-phosphonooxy-1-azabicyc
Specifications & Purity	biotechnology Grade
Storage Temp	Store at 2-8°C
Shipped In	Wet ice
Grade	BioReagent
Product Description	Product Introduction Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. Application Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Names and Identifiers

Enzyme Commission Number	3.4.21.64
IUPAC Name	(4-nitrophenyl)methyl (4R,5S,6S)-6-[(1R)-1-hydroxyethyl]-4-methyl-7-oxo-3,4-diphenoxy-5-phosphonooxy-1-azabicyclo[3.2.0]hept-2-ene-2-carboxylate
INCHI	InChI=1S/C29H27N2O12P/c1-18(32)23-26(33)30-24(27(34)40-17-19-13-15-20(16-14-19)31(35)36)25(41-21-9-5-3-6-10-21)28(2,29(23,30)43-44(37,38)39)42-22-11-7-4-8-12-22/h3-16,18,23,32H,17H2,1-2H3,(H2,37,38,39)/t18-,23-,28-,29+/m1/s1
InChi Key	OUPAKHMWUJJXOM-ANOMEZDXSA-N
Canonical SMILES	CC(C1C(=O)N2C1(C(C(=C2C(=O)OCC3=CC=C(C=C3)[N+](=O)[O-])OC4=CC=CC=C4)(C)OC5=CC=CC=C5)OP(=O)(O)O)O
Isomeric SMILES	C[C@H]([C@@H]1C(=O)N2[C@]1([C@](C(=C2C(=O)OCC3=CC=C(C=C3)[N+](=O)[O-])OC4=CC=CC=C4)(C)OC5=CC=CC=C5)OP(=O)(O)O)O
WGK Germany	1
PubChem CID	118855431

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number	Certificate Type	Date	Item
I2118428	Certificate of Analysis	Apr 17, 2023	P274341
I2118430	Certificate of Analysis	Apr 17, 2023	P274341
I2118431	Certificate of Analysis	Apr 17, 2023	P274341
I2118432	Certificate of Analysis	Apr 17, 2023	P274341

Safety and Hazards(GHS)

Pictogram(s)	GHS08, GHS07
Signal	Danger
Hazard Statements	H315:Causes skin irritation H319:Causes serious eye irritation H335:May cause respiratory irritation H317:May cause an allergic skin reaction H334:May cause allergy or asthma symptoms or breathing difficulties if inhaled
Precautionary Statements	P261:Avoid breathing dust/fume/gas/mist/vapors/spray. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of water. P321:Specific treatment (see ... on this label). P405:Store locked up. P501:Dispose of contents/container to ... P264:Wash hands [and …] thoroughly after handling. P284:[In case of inadequate ventilation] Wear respiratory protection. P271:Use only outdoors or in a well-ventilated area. P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing. P403+P233:Store in a well-ventilated place. Keep container tightly closed. P272:Contaminated work clothing should not be allowed out of the workplace. P333+P313:IF SKIN irritation or rash occurs: Get medical advice/attention. P362+P364:Take off contaminated clothing and wash it before reuse. P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes. P337+P317:If eye irritation persists: Get medical help. P332+P317:If skin irritation occurs: Get medical help. P319:Get medical help if you feel unwell. P342+P316:If experiencing respiratory symptoms: Get emergence medical help immediately.
WGK Germany	1

FAQs and Articles

Citations of This Product

1. Wenlong Pang, Bin Li, Yufeng Wu, Shaonan Tian, Yu Zhang, Jun Yang. (2023) Optimization of degradation behavior and conditions for the protease K of polylactic acid films by simulation. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 253 (15): (127496). [PMID:37858641]
2. Kairui Duan, Nabila Mehwish, Mengdie Xu, Hu Zhu, Jiajun Hu, Mian Lin, Lu Yu, Bae Hoon Lee. (2023) Autoclavable Albumin-Based Cryogels with Uncompromising Properties. Gels, 9 (9): (712). [PMID:37754393]
3. Jie Zhang, Zhe Kong, Xiangyao Li, Chuang Zhang, Kaijun Xu, Guiqing Cheng, Zhongwei Wang, Qing Yu. (2023) The influence of Scutellaria baicalensis extract obtained from traditional Chinese herbal medicine on the thermo-oxidative degradation and stabilization of plasticized polylactic acid. POLYMER ENGINEERING AND SCIENCE, 63 (9): (3105-3115). [PMID:]
4. Zixuan Ding, Peilin Wang, Zhenrun Li, Yupeng Guo, Qiang Ma. (2023) Sulfur dots/Au@Ag nanorods array-based polarized ECL sensor for the detection of thyroid cancer biomarker. TALANTA, 265 (124925). [PMID:37437396]
5. Qingxu Song, Gongde Lan, Qian Li, Yang Lin, Xin Chen, Hong Liu, Yufeng Cheng, Yuxia Luan. (2023) A hydrogel-enabled cascade amplification of radiosensitization and immune activation for cancer radiotherapy. CHEMICAL ENGINEERING JOURNAL, 462 (142201). [PMID:]
6. Yanhua Xu, Huiyan Dong, Chenxi Liu, Haiwei Lou, Renyong Zhao. (2023) Efficient Aflatoxin B1 degradation by a novel isolate, Pseudomonas aeruginosa M-4. FOOD CONTROL, 149 (109679). [PMID:]
7. Lihuang Wu, Yuqi Wang, Xinyue Zhao, Hongli Mao, Zhongwei Gu. (2023) Investigating the Biodegradation Mechanism of Poly(trimethylene carbonate): Macrophage-Mediated Erosion by Secreting Lipase. BIOMACROMOLECULES, 24 (2): (921–928). [PMID:36644840]
8. Guoli Xue, Yanjun Qu, Dan Wu, Shuyuan Huang, Yuqing Che, Jing Yu, Ping Song. (2023) Biodegradation of Aflatoxin B1 in the Baijiu Brewing Process by Bacillus cereus. Toxins, 15 (1): (65). [PMID:36668884]
9. Xiangpeng Han, Mengyu Zhang, Jiayi Peng, Jinsong Wu, Qingping Zhong. (2023) Purification and characterization of a novel bacteriocin from Lactiplantibacillus plantarum Z057, and its antibacterial and antibiofilm activities against Vibrio parahaemolyticus. LWT-FOOD SCIENCE AND TECHNOLOGY, 173 (114358). [PMID:]
10. Tingting Wu, Xushun Liu, Hanjun Chen, Ying Liu, Yu Cao. (2023) An in situ exosomal miRNA sensing biochip based on multi-branched localized catalytic hairpin assembly and photonic crystals. BIOSENSORS & BIOELECTRONICS, 222 (115013). [PMID:36529054]
11. Mengdie Xu, Nabila Mehwish, Bae Hoon Lee. (2022) Facile Fabrication of Transparent and Opaque Albumin Methacryloyl Gels with Highly Improved Mechanical Properties and Controlled Pore Structures. Gels, 8 (6): (367). [PMID:35735711]
12. Fangjun Liu, Dun Wang, Jiaqi Wang, Liwei Ma, Cuiyun Yu, Hua Wei. (2022) Construction of Enzyme-Responsive Micelles Based on Theranostic Zwitterionic Conjugated Bottlebrush Copolymers with Brush-on-Brush Architecture for Cell Imaging and Anticancer Drug Delivery. MOLECULES, 27 (9): (3016). [PMID:35566368]
13. Fangjun Liu, Dun Wang, Miao Zhang, Liwei Ma, Cui-Yun Yu, Hua Wei. (2022) Synthesis of enzyme-responsive theranostic amphiphilic conjugated bottlebrush copolymers for enhanced anticancer drug delivery. Acta Biomaterialia, 144 (15). [PMID:35306183]
14. Xiaoping Wu, Qiujie Pang, Fuxiang Zhi, Xiaohong Mao, Yingbin Hu. (2022) Overexpression of long non-coding RNA ACTA2-AS1 inhibits the viability, proliferation, migration and invasion of colorectal cancer cells. TISSUE & CELL, 76 (101769). [PMID:35325674]
15. Tao Zhang, Dekun Zhang, Hongtao Liu, Kai Chen. (2021) Quantitative analysis and degradation mechanisms of different protein degradation methods. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 110 (5): (1034-1043). [PMID:34842354]

References

1. Wenlong Pang, Bin Li, Yufeng Wu, Shaonan Tian, Yu Zhang, Jun Yang. (2023) Optimization of degradation behavior and conditions for the protease K of polylactic acid films by simulation. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 253 (15): (127496). [PMID:37858641]
2. Kairui Duan, Nabila Mehwish, Mengdie Xu, Hu Zhu, Jiajun Hu, Mian Lin, Lu Yu, Bae Hoon Lee. (2023) Autoclavable Albumin-Based Cryogels with Uncompromising Properties. Gels, 9 (9): (712). [PMID:37754393]
3. Jie Zhang, Zhe Kong, Xiangyao Li, Chuang Zhang, Kaijun Xu, Guiqing Cheng, Zhongwei Wang, Qing Yu. (2023) The influence of Scutellaria baicalensis extract obtained from traditional Chinese herbal medicine on the thermo-oxidative degradation and stabilization of plasticized polylactic acid. POLYMER ENGINEERING AND SCIENCE, 63 (9): (3105-3115). [PMID:]
4. Zixuan Ding, Peilin Wang, Zhenrun Li, Yupeng Guo, Qiang Ma. (2023) Sulfur dots/Au@Ag nanorods array-based polarized ECL sensor for the detection of thyroid cancer biomarker. TALANTA, 265 (124925). [PMID:37437396]
5. Qingxu Song, Gongde Lan, Qian Li, Yang Lin, Xin Chen, Hong Liu, Yufeng Cheng, Yuxia Luan. (2023) A hydrogel-enabled cascade amplification of radiosensitization and immune activation for cancer radiotherapy. CHEMICAL ENGINEERING JOURNAL, 462 (142201). [PMID:]
6. Yanhua Xu, Huiyan Dong, Chenxi Liu, Haiwei Lou, Renyong Zhao. (2023) Efficient Aflatoxin B1 degradation by a novel isolate, Pseudomonas aeruginosa M-4. FOOD CONTROL, 149 (109679). [PMID:]
7. Lihuang Wu, Yuqi Wang, Xinyue Zhao, Hongli Mao, Zhongwei Gu. (2023) Investigating the Biodegradation Mechanism of Poly(trimethylene carbonate): Macrophage-Mediated Erosion by Secreting Lipase. BIOMACROMOLECULES, 24 (2): (921–928). [PMID:36644840]
8. Guoli Xue, Yanjun Qu, Dan Wu, Shuyuan Huang, Yuqing Che, Jing Yu, Ping Song. (2023) Biodegradation of Aflatoxin B1 in the Baijiu Brewing Process by Bacillus cereus. Toxins, 15 (1): (65). [PMID:36668884]
9. Xiangpeng Han, Mengyu Zhang, Jiayi Peng, Jinsong Wu, Qingping Zhong. (2023) Purification and characterization of a novel bacteriocin from Lactiplantibacillus plantarum Z057, and its antibacterial and antibiofilm activities against Vibrio parahaemolyticus. LWT-FOOD SCIENCE AND TECHNOLOGY, 173 (114358). [PMID:]
10. Tingting Wu, Xushun Liu, Hanjun Chen, Ying Liu, Yu Cao. (2023) An in situ exosomal miRNA sensing biochip based on multi-branched localized catalytic hairpin assembly and photonic crystals. BIOSENSORS & BIOELECTRONICS, 222 (115013). [PMID:36529054]
11. Mengdie Xu, Nabila Mehwish, Bae Hoon Lee. (2022) Facile Fabrication of Transparent and Opaque Albumin Methacryloyl Gels with Highly Improved Mechanical Properties and Controlled Pore Structures. Gels, 8 (6): (367). [PMID:35735711]
12. Fangjun Liu, Dun Wang, Jiaqi Wang, Liwei Ma, Cuiyun Yu, Hua Wei. (2022) Construction of Enzyme-Responsive Micelles Based on Theranostic Zwitterionic Conjugated Bottlebrush Copolymers with Brush-on-Brush Architecture for Cell Imaging and Anticancer Drug Delivery. MOLECULES, 27 (9): (3016). [PMID:35566368]
13. Fangjun Liu, Dun Wang, Miao Zhang, Liwei Ma, Cui-Yun Yu, Hua Wei. (2022) Synthesis of enzyme-responsive theranostic amphiphilic conjugated bottlebrush copolymers for enhanced anticancer drug delivery. Acta Biomaterialia, 144 (15). [PMID:35306183]
14. Xiaoping Wu, Qiujie Pang, Fuxiang Zhi, Xiaohong Mao, Yingbin Hu. (2022) Overexpression of long non-coding RNA ACTA2-AS1 inhibits the viability, proliferation, migration and invasion of colorectal cancer cells. TISSUE & CELL, 76 (101769). [PMID:35325674]
15. Tao Zhang, Dekun Zhang, Hongtao Liu, Kai Chen. (2021) Quantitative analysis and degradation mechanisms of different protein degradation methods. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 110 (5): (1034-1043). [PMID:34842354]

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SKU	Size	Availability	Price
P274341-25mg	25mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$171.90
P274341-100mg	100mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$289.90
P274341-500mg	500mg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$1,329.90
P274341-1g	1g	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$2,121.90

Proteinase K - biotechnology Grade, high purity , CAS No.39450-01-6