Pyrophosphatase, Inorganic （E.coli）

Item Number

P406590

• Storage Temp: Store at -20°C
• Shipped In: Ice chest + Ice pads
• Grade: EnzymoPure™

Product Description

Inorganic pyrophosphatase (PPase) can catalyze the hydrolysis of inorganic pyrophosphate to orthophosphate: P2O74-+H2O+PPase→2HPO42-. Used in nucleic acid amplification experiments, PPase can hydrolyze the inorganic pyrophosphate generated by the reaction to avoid its inhibition of the reaction system. Under natural conditions, inorganic pyrophosphatase can provide thermodynamic power for the biosynthesis of protein, RNA and DNA, and play a role in the process of lipid metabolism, calcium absorption, and bone formation.

Features

Hydrolyzed pyrophosphate;

DNA synthesis: significantly enhance the ability of DNA replication;

RNA synthesis: increase RNA yield in in vitro transcription reaction;

Thermal stability: The best reaction temperature is 25°C, 65°C for 10 minutes can inactivate the enzyme.

Recommendations

1) The enzyme is active in a variety of reaction buffers. Usually, the enzyme can be directly added in experiments such as HDA amplification and LAMP amplification;

2) The dosage of the enzyme needs to be optimized in different experiments, usually adjusted at a concentration of 0.05~1U/ml;

3) The optimal reaction temperature of the enzyme is 25°C, and it is active at 16~37°C, and it can be inactivated at 65°C for 10 minutes.