Pyrophosphate Assay Kit - sufficient for 200fluorometrictests (Blue fluorescence), high purity

Item Number

P486465

• Synonyms: Fluorimetric Pyrophosphate Assay Kit
• Specifications & Purity: sufficient for 200fluorometrictests (Blue fluorescence)
• Storage Temp: Protected from light,Store at -20°C
• Shipped In: Ice chest + Ice pads

Product Description

Pyrophosphate (PPi) is produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters. Pyrophosphate Assay Kit provides the most robust spectrophotometric method for measuring pyrophosphate. This kit uses proprietary fluorogenic pyrophosphate sensor that has its fluorescence intensity proportionally dependent upon the concentration of pyrophosphate. Our assay is much easier and more robust than the enzyme-coupling pyrophosphate methods that require at least two enzymes for their pyrophosphate detections. The kit provides all the essential components for assaying pyrophosphate. This kit has been successfully used in high throughput screening (HTS). 

Platform
Fluorescence microplate reader  
Excitation 316 nm
Emission 456 nm
Cutoff 420 nm
Recommended plate Solid black
Components
Component A: Assay Buffer 1 bottle (25 mL)
Component B: PPi Sensor 1 vial (lyophilized powder)
Component C: Pyrophosphate Standard 1 vial (1 mL, 50 mM)
Component D: DMSO 1 vial (200 µL)