Quick Hybridization Solution

Item Number

Q743280

• Stability And Storage: Store at room temperature or 4℃, valid for at least one year.
• Storage Temp: Store at 2-8°C
• Shipped In: Wet ice

Product Description

Aladdin's Quick Hybridization Solution is designed for rapid and efficient hybridization of RNA/DNA with RNA, DNA, or oligonucleotide probes in Northern and Southern blot experiments. This product is sterile and free of DNase/RNase/Protease. It can be used in conjunction with Aladdin's Biotin Northern Blot Kit and Biotin Northern Blot Kit (for Small RNA) .This product contains a unique hybridization accelerator, which greatly enhances the hybridization speed and the detection sensitivity. Usually, hybridization for only 2 hours can achieve the same effect as overnight hybridization. For example, when using this product in Northern blot assay of tRNA-Ala in total RNA from mouse (Figure 1), a good detection signal similar to overnight hybridization was obtained with only 1ng of total RNA after a 2-hour hybridization.Figure 1. Northern blot assay of tRNA-Ala in total RNA from mouse liver tissues using Aladdin's Quick Hybridization Solution . The 3'-biotinylated oligo probe (5'-TGGAGATGCCGGGGATCGAACC-Biotin-3) was used to hybridize the mouse-tRNA-Ala-TGC-3-1 for 2h and overnight, respectively. This figure is for reference only, which may vary due to different experimental conditions.The Northern assay was invented in 1977 by James Alwine, David Kemp and George Stark at Stanford University and can be used to detect the size, abundance and migration position of RNA after electrophoresis [1, 2]. Since the DNA Blot was named "Southern Blot" after its inventor Edwin Southern, the RNA Blot was named "Northern Blot" [1, 2]. Northern Blot consists of the following main steps

Precautions：

Hyb™ Quick Hybridization Solution may precipitate at lower temperatures. If precipitation occurs, it can be incubated in a 40-50℃ water bath to dissolve the precipitates completely before use.Avoid RNase contamination during operation. Wear a disposable mask when using this product and minimize its exposure to air. After use, the lid should be immediately covered and tightened. This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.This product can be used for hybridization of RNA or DNA samples on nylon membranes with DNA, RNA, or Oligonucleotide probes. It can also be used for pre-hybridization. Generally, 10ml of Quick Hybridization Solution is needed for every 100cm2 of nylon membrane.2.For detailed procedures of Northern blot, please refer to the manual of Quick Hybridization Solution500mlR0251-500mlLow Stringency Wash Solution (DNase/RNase free)500mlR0253-500mlHigh Stringency Wash Solution (DNase/RNase free)500mlR0262-250mlNorthern Blot Stripping Solution (Mild)250mlR0266-250mlNorthern Blot Stripping Solution (Moderate)250mlR0268-250mlNorthern Blot Stripping Solution (Harsh)250mlA0303HRP-labeled Streptavidin0.2mlP0018F Ultrapure Water (DNase/RNase-Free, Sterile)100/500mlR0021DEPC-water (DNase、RNase free)100mlR0022DEPC-water (DNase、RNase free)500mlST004LAgarose50gST004B40% Acr-Bis (19:1, Nuclease free)100mlR0123RNase and DNase Away250mlR0125RNase, DNase and DNA Away250mlR0127RNase, DNase, DNA and RNA Away250ml