mAb (1596CT245.254.49); Mouse anti Human RAB27B Antibody; WB, Flow; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab124166-10μl | 10μl | In stock | $69.90 | |
| Ab124166-50μl | 50μl | In stock | $189.90 | |
| Ab124166-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab124166-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,309.90 |
mAb (1596CT245.254.49); Mouse anti Human RAB27B Antibody; WB, Flow; Unconjugated
| Product Name | RAB27B Mouse mAb - Primary antibody, specific to RAB27B, Mouse IgG1 |
|---|---|
| Synonyms | C25KG antibody | Rab27b antibody | RAB27B member RAS oncogene family antibody | RAS associated protein RAB27B antibody | Ras related protein Rab 27b antibody | Ras related protein Rab27b antibody | Ras-related protein Rab-27B antibody | RB27B_HUMAN antibo |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Host species | Mouse |
| Specificity | RAB27B |
| Immunogen | Recombinant protein of human RAB27B (AA 1-218) |
| Positive Control | WB: A431 and C6 cell lysates. Flow: A431 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description | Mouse anti Human RAB27B Antibody, Monoclonal (1596CT245.254.49), could be used for WB, Flow and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
RAB27B Mouse mAb (Ab124166) - Western Blot
All lanes: RAB27B Mouse mAb (Ab124166) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 3.0 s
RAB27B Mouse mAb (Ab124166) - Flow Cytometry
Overlay histogram showing A431 cells stained with RAB27B Mouse mAb (Ab124166) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by RAB27B Mouse mAb (Ab124166) (1/25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| Flow | 1/25 |
| WB | 1/2000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303557 | Certificate of Analysis | Mar 21, 2024 | Ab124166 |
| ZJ24F0303556 | Certificate of Analysis | Mar 21, 2024 | Ab124166 |
