Rabbit Anti-Goat IgG H&L Antibody - Secondary antibody, specific to Goat IgG, Rabbit IgG
Specifications & Purity
ExactAb™, Validated, Carrier Free, Azide Free, High performance
Host species
Rabbit
Immunogen
Goat IgG
Positive Control
Lot by Lot
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description
Rabbit Anti-Goat IgG H&L Antibody, Secondary antibody, could be used for WB, CLIA, LF, GICA, EIA and so on.
WB: 1/500 - 1/10000
CLIA: 0.5µg/mL-10µg/mL
LF/ GICA: 0.5mg/mL-2.0mg/mL
EIA: 1/1000 - 1/50000
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
24.9 kDa (Light Chain) & 50.3 kDa (Heavy Chain), under reducing conditions; 131.0 kDa, under non-reducing conditions.
Purification Method
Antigen affinity chromatography
Form
Liquid
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Rabbit Anti-Goat IgG H&L Antibody (Ab141541) - ELISA Rabbit Anti-Goat IgG H&L Antibody (Ab141541) was tested by ELISA. The plate was coated with 1.0 μg/mL Goat IgG (Ab170217). Rabbit Anti-Goat IgG H&L Antibody was biotinylated then added at a series of different dilution ratios. Streptavidin-HRP (np156148) was used at 1/10000 dilution for this ELISA.
Rabbit Anti-Goat IgG H&L Antibody (Ab141541) - ELISA Cross-reactivity of Rabbit Anti-Goat IgG H&L Antibody (Ab141541) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Rabbit Anti-Goat IgG H&L Antibody was biotinylated then added at different dilution ratio followed by incubation for 1h. Streptavidin-HRP (np156148) was used at 1/10000 dilution for this ELISA.
Application
Application
Dilution info
CLIA
0.5-10 µg/mL
LF/GICA
0.5-2.0 mg/mL
EIA
1/1000 - 1/50000
WB
1/5000 – 1/10000
Certificates
Certificate of Analysis(COA)
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