Bioactivity: 1/4 dilution versus activated NHS assayed by immunodiffusion 1/500 to 1/1000 effective concentrations in Western Blots 1/500 to 1/2000 effective concentrations in ELISA
Item Number
R501101
Grouped product items
SKU
Size
Availability
Price
Qty
R501101-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
Extinction Coeff：A280 nm = 1.0 at 1.0 mg/mL Specificity: Monospecific for C5a and C5adesArg in human plasma
General Description
Product is whole polyclonal antiserum from rabbits immunized with highly
purified human complement protein. Product is not a purified IgG fraction. Rabbits are maintained in FDA certified facilities.
Physical Characteristics & Structure
Antibodies present in the antisera are primarily IgG
Applications
Recognizes rC5a, human C5a and human C5adesArg. Western Blots: Effective at dilutions 1/500 to 1/1000 depending on conditions.Most effective against non-reduced antigen. ELISA: Effective at dilutions 1/500 to 1/2000 depending on conditions. Immunodiffusion: Effective against 50 µg/ml C5a at 1/4 dilution.
Specifications & Purity
>40 mg/mL total protein concentration
Bioactivity
1/4 dilution versus activated NHS assayed by immunodiffusion 1/500 to 1/1000 effective concentrations in Western Blots 1/500 to 1/2000 effective concentrations in ELISA
Source
Rabbits (Oryctolagus cuniculus) immunized with highly purified human complement protein
