Purified rabbit polyclonal antibody, suitable for LF, CLIA, EIA, WB, IHC and other immune detection platforms and other suitable application scenarios. Antigen affinity chromatography, purity greater than 95%, buffer system 0.01M PBS (pH7.4), 0.2μm filtration, no preservatives added.
Product Properties
Purification Method
antigen affinity chromatography
Form
liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Images
Rabbit anti-mouse IgG antibody (Ab170451) - ELISA Rabbit anti-mouse IgG antibody (Ab170451) was tested by ELISA. The plate was coated with different amounts of mouse IgG (Ab170221) (3-fold started from 100 ng/mL). Rabbit anti-mouse IgG antibody was then added at a series of different dilution ratios. Anti-Rabbit IgG H&L (Biotin) (Ab136895) was used at 1/10000 dilution as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/10000 dilution for this ELISA.
Rabbit anti-mouse IgG antibody (Ab170451) - ELISA Cross-reactivity of Rabbit anti-mouse IgG antibody (Ab170451) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Rabbit anti-mouse IgG antibody was then added at different dilution ratio followed by incubation for 1h. Donkey Anti-Rabbit IgG H&L (Biotin) (Ab136895) was used at 1/10000 dilution as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/10000 dilution for this ELISA. For the batch tested, Rabbit anti-mouse IgG antibody (Ab170451) showed a cross-reactivity with Human IgG when the dilution ratio of Rabbit anti-mouse IgG antibody is less than 1/64000.
Certificates
Certificate of Analysis(COA)
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ZJ23F0800817