Secondary Antibody; Rabbit Anti-Mouse IgG (Biotin); WB, ELISA, IF
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab176439-100μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 100μg | In stock | $19.90 | |
| Ab176439-1mg | 1mg | In stock | $119.90 |
Secondary Antibody; Rabbit Anti-Mouse IgG (Biotin); WB, ELISA, IF
| Product Name | Rabbit Anti-Mouse IgG (Biotin) - Secondary antibody, specific to Mouse IgG |
|---|---|
| Synonyms | Rabbit Anti-Mouse IgG/Biotin; Rabbit Anti-Mouse IgG-Biotin; Anti-Mouse IgG-Biotin antibody produced in rabbit |
| Specifications & Purity | ExactAb™, Validated, Azide Free, High performance, 1.0 mg/mL |
| Host species | Rabbit |
| Conjugation | HRP |
| Grade | Azide Free, ExactAb™, High Performance, Validated |
| Product Description | Application |
| Purification Method | Antigen affinity purified |
|---|---|
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing |
| Shipped In | Wet ice |
| Stability And Storage | Store at 4℃. Avoid freeze/thaw cycle. Store in the dark. |
Rabbit Anti-Mouse IgG (Biotin) (Ab176439) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Rabbit Anti-Mouse IgG (Biotin) (Ab176439) at 1/40000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 8.4 s
Rabbit Anti-Mouse IgG (Biotin) (Ab176439) - ELISA
Rabbit Anti-Mouse IgG (Biotin) (Ab176439) was tested by direct ELISA. The plate was coated with different amounts of Mouse IgG (Ab170221). A serial dilution of Rabbit Anti-Mouse IgG (Biotin) (Ab176439) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.
| Application | Dilution info |
|---|---|
| WB | 1/10000-1/200000 |
| ELISA | 1:10000-1:200000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1202219 | Certificate of Analysis | Dec 26, 2023 | Ab176439 |
| ZJ23F1202220 | Certificate of Analysis | Dec 26, 2023 | Ab176439 |
| ZJ23R1100137 | Certificate of Analysis | Nov 15, 2023 | Ab176439 |
