Raf1-RBD Agarose

Item Number

R749796

• Product Name: Raf1-RBD Agarose

Product Description

Aladdin's Raf1-RBD Agarose is generated by combining GST-tagged human Raf1 RBD domain recombinantly expressed in E. coli with glutathione agarose . This product can be used to bind, precipitate, or pull down active Ras proteins (including NRas, HRas and KRas), to investigate the proportion of active Ras to inactive Ras in vitro, and can also be used for quantitative analysis of active Ras proteins in tissue or cell lysate.Please refer to Figure 1 for the working principle of this product.Figure 1. Schematic diagram of the working principle of Aladdin's Raf1-RBD Agarose .Raf1 is a downstream effector protein that interacts with the Ras subfamily (including NRas, HRas and KRas) and plays an important role in cell proliferation, differentiation, apoptosis, survival and carcinogenesis. The full-length Raf1 protein comprises 648 amino acid residues, with a theoretical molecular weight of 73kDa. It contains four domains

Precautions：

Store at -20℃ or -80℃ upon receipt. After first thawing, aliquot this product to avoid repeated freeze-thaw cycles.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

For the usage of this product, please refer to the manual of Active Ras Pull-down and Detection Kit (, P2433).References:1. Tran TH, Chan AH, Young LC, Bindu L, Neale C. Nat Commun. 2021. 12(1):1176.2. Vernoud V, Horton AC, Yang Z, Nielsen E. Plant Physiol. 2003. 131(3):1191-208.3. Ito H, Morishita R, Nagata KI. Int J Mol Sci. 2018. 19(7):2121.4. Goitre L, Trapani E, Trabalzini L, Retta SF. Methods Mol Biol. 2014. 1120:1-18.5. Bos JL. Cancer Res. 1989. 49(17):4682-9.6. Mann D, Güldenhaupt J, Schartner J, Gerwert K, Kötting C. J Biol Chem. 2018. 293(11):3871-9.