The Ramos whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. Ramos cell line was derived from a caucasian Burkitt's lymphoma which does not possess the Epstein Barr Virus (EBV) genome. EBV infectability and permanent conversion into EBV positive sub-lines is possible by in vitro infection. The cells have B lymphocyte characteristics, with surface associated mu and kappa chains. Cells are used as model of B lymphocytes and for apoptosis studies.
Product Description
Mycoplasma free
Specifications & Purity
Carrier Free, Azide Free, 200μg, Mycoplasma free
Images
Ramos Whole Cell Lysate (cl155908) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897) Lane 2: MOLT-4 Whole Cell Lysate (cl155905) Lane 3: LNCaP Whole Cell Lysate (cl155902) Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa
Ramos Whole Cell Lysate (cl155908) - Western Blot All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897) Lane 2: MOLT-4 Whole Cell Lysate (cl155905) Lane 3: LNCaP Whole Cell Lysate (cl155902) Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa Observed band size: 29 kDa
Ramos Whole Cell Lysate (cl155908) - Western Blot All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897) Lane 2: MOLT-4 Whole Cell Lysate (cl155905) Lane 3: LNCaP Whole Cell Lysate (cl155902) Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa Observed band size: 58 kDa
Product Specifications
Form
Lyophilized
Concentration
200μg, Mycoplasma free
Reconstitution
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. Lyophilized contents may appear as either a translucent film or a white po
Storage Temp
Store at -80°C
Shipped In
Ice chest + Ice pads
Stability And Storage
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
Certificates
Certificate of Analysis(COA)
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
ZJ23F0700551