Rat IgG Isotype Control Antibody (Biotin) - Isotype Control Antibody, IgG

    Application:
  • Flow Cytometry
Features and benefits
  • Host species: Rat
  • Isotype: Rat IgG
  • Conjugation: Biotin
Item Number
Ab180310
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab180310-10μg (Trial Size)
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10μg
In stock
$19.90
Ab180310-100μg
100μg
In stock
$28.90
Ab180310-1mg
1mg
Available within 8-12 weeks(?)
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$139.90

pAb; Isotype Control Antibody; Rat IgG (Biotin); Flow, ELISA

View related series
Isotype Control Antibody Rat IgG

Basic Description

Product NameRat IgG Isotype Control Antibody (Biotin) - Isotype Control Antibody, IgG
SynonymsRat IgG | Biotin-conjugated Rat Polyclonal antibody | Biotin Rat IgG - Isotype Control
Specifications & PurityExactAb™, Validated, Azide Free, 0.5 mg/mL
Host speciesRat
ConjugationBiotin
GradeAzide Free, ExactAb™, Validated
Product Description

Application:

Flow: ≤1μg/test

For flow cytometric staining, the suggested use of this reagent is ≤1 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Product Properties

IsotypeIgG
SDS-PAGE150 kDa
Purification MethodAffinity chromatography
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at 2-8°C,Protected from light,Freeze prohibited
Shipped InWet ice
Stability And StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - ELISA
Rat IgG Isotype Control Antibody (Biotin) (Ab180310) was tested by direct ELISA. The plate was coated with Goat Anti-Rat IgG H&L Antibody (Ab138352). A serial dilution of Rat IgG Isotype Control Antibody (Biotin) (Ab180310) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - Flow Cytometry
Flow cytometry analysis of CD16/CD32 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD16/CD32 Rat mAb (Biotin) (Ab179681) at 0.03μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

ApplicationDilution info
Flow Cytometry≤1.0μg/ test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

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≤1.0μg/ test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303390Certificate of AnalysisMar 19, 2024 Ab180310
ZJ24F0303391Certificate of AnalysisMar 19, 2024 Ab180310
ZJ24R0200271Certificate of AnalysisFeb 02, 2024 Ab180310

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