pAb; Isotype Control Antibody; Rat IgG (Biotin); Flow, ELISA
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab180310-10μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μg | In stock | $19.90 | |
| Ab180310-100μg | 100μg | In stock | $28.90 | |
| Ab180310-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $139.90 |
pAb; Isotype Control Antibody; Rat IgG (Biotin); Flow, ELISA
| Product Name | Rat IgG Isotype Control Antibody (Biotin) - Isotype Control Antibody, IgG |
|---|---|
| Synonyms | Rat IgG | Biotin-conjugated Rat Polyclonal antibody | Biotin Rat IgG - Isotype Control |
| Specifications & Purity | ExactAb™, Validated, Azide Free, 0.5 mg/mL |
| Host species | Rat |
| Conjugation | Biotin |
| Grade | Azide Free, ExactAb™, Validated |
| Product Description | Application: Flow: ≤1μg/test For flow cytometric staining, the suggested use of this reagent is ≤1 μg per million cells in 100 µL staining volume or 100 µL of whole blood. |
| Isotype | IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Affinity chromatography |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Freeze prohibited |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - ELISA
Rat IgG Isotype Control Antibody (Biotin) (Ab180310) was tested by direct ELISA. The plate was coated with Goat Anti-Rat IgG H&L Antibody (Ab138352). A serial dilution of Rat IgG Isotype Control Antibody (Biotin) (Ab180310) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.
Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - Flow Cytometry
Flow cytometry analysis of CD16/CD32 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD16/CD32 Rat mAb (Biotin) (Ab179681) at 0.03μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤1.0μg/ test |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303390 | Certificate of Analysis | Mar 19, 2024 | Ab180310 |
| ZJ24F0303391 | Certificate of Analysis | Mar 19, 2024 | Ab180310 |
| ZJ24R0200271 | Certificate of Analysis | Feb 02, 2024 | Ab180310 |
