Recombinant A-Raf Antibody - Primary antibody, specific to ARAF, Rabbit IgG
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.5 mg/mL
Host species
Rabbit
Specificity
ARAF
Immunogen
Recombinant protein of human A RAF (AA 166-290)
Positive Control
WB: HeLa, A431, A375, Raji and Ramos cell lysates. IHC: Human lung cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human A-Raf Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant A-Raf Antibody (Ab086857) - Western Blot All lanes: Recombinant A-Raf Antibody (Ab086857) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 80.0 s
Recombinant A-Raf Antibody (Ab086857) - Western Blot All lanes: Recombinant A-Raf Antibody (Ab086857) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 100.0 s
Recombinant A-Raf Antibody (Ab086857) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant A-Raf Antibody (Ab086857). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.