Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free!
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab088446-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$207.90
Ab088446-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$297.90
Ab088446-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
ExactAb™, Validated, Recombinant, High performance, 0.05 mg/mL
Host species
Rabbit
Specificity
ACTA2
Immunogen
A synthetic peptide corresponding to residues on the N-terminus of human Alpha Actin 2 was used as an immunogen.
Positive Control
WB: Hela, HEK293, LNCap, Raji, Hep G2, A431, Jurkat, HUVEC, C6, NIH/3T3 cell lyeates. IHC: Placenta. Flow: HeLa cells. IF/ICC: HeLa cells.
Conjugate
Unconjugated
Grade
ExactAb™, High performance, Recombinant
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.05 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Dry ice
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant ACTA2 Antibody (Ab088446) - Western Blot All lanes: Recombinant ACTA2 Antibody (Ab088446) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 44 kDa Exposure time: 80.0 s
Recombinant ACTA2 Antibody (Ab088446) - IF IF analysis of ACTA2 (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant ACTA2 Antibody (Ab088446) at 1/100 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant ACTA2 Antibody (Ab088446) - IHC Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of placenta tissue labelling ACTA2 with Recombinant ACTA2 Antibody (Ab088446) at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant ACTA2 Antibody (Ab088446) - Flow Cytometry Overlay histogram showing HeLa cells stained with Recombinant ACTA2 Antibody (Ab088446) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant ACTA2 Antibody (Ab088446) (1/100 dilution) in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at 4°C. Unlabeled sample (Green) was used as a control.