Recombinant (R03-8C2); Rabbit anti Human ALIX Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab088156-10μl (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab088156-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab088156-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab088156-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant (R03-8C2); Rabbit anti Human ALIX Antibody; WB; Unconjugated
| Product Name | Recombinant ALIX Antibody - Primary antibody, specific to PDCD6IP, Rabbit IgG |
|---|---|
| Synonyms | PDC6I_HUMAN | Programmed cell death 6-interacting protein | AIP1 | ALIX | KIAA1375 | PDCD6-interacting protein | ALG-2-interacting protein 1 | ALG-2-interacting protein X | Hp95 | AIP1 antibody | ALG 2 interacting protein 1 antibody | ALG-2-interacting pr |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.2 mg/mL |
| Host species | Rabbit |
| Specificity | PDCD6IP |
| Immunogen | A synthetic peptide derived from human ALIX (AA 3-102) |
| Positive Control | WB: HeLa, MCF7, Jurkat, A549, K562, NIH/3T3 and C6 cell lysates. |
| Conjugate | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Dry ice |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant ALIX Antibody (Ab088156) - Western Blot
All lanes: Recombinant ALIX Antibody (Ab088156) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 96 kDa
Observed band size: 81 kDa
Exposure time: 7.0 s
Recombinant ALIX Antibody (Ab088156) - Western Blot
All lanes: Recombinant ALIX Antibody (Ab088156) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 96 kDa
Observed band size: 80, 100 kDa
Exposure time: 25.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Notes |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303220 | Certificate of Analysis | Mar 14, 2024 | Ab088156 |
| ZJ24F0303221 | Certificate of Analysis | Mar 14, 2024 | Ab088156 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Nagase, T T, Kikuno, R R, Ishikawa, K I KI, Hirosawa, M M and Ohara, O O.. (2000) Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro.. DNA research : an international journal for rapid publication of reports on genes and genomes, (28): [PMID:10718198] |
| 4. Wu, Y Y and 6 more authors.. (2001) Overexpression of Hp95 induces G1 phase arrest in confluent HeLa cells.. Differentiation; research in biological diversity, [PMID:11683497] |
| 5. Katoh, Keiichi K and 7 more authors.. (2003) The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting.. The Journal of biological chemistry, (3): [PMID:12860994] |
| 6. Strack, Bettina B, Calistri, Arianna A, Craig, Stewart S, Popova, Elena E and Göttlinger, Heinrich G HG.. (2003) AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding.. Cell, (19): [PMID:14505569] |
| 7. von Schwedler, Uta K UK and 14 more authors.. (2003) The protein network of HIV budding.. Cell, (19): [PMID:14505570] |
| 8. Martin-Serrano, Juan J, Yarovoy, Anton A, Perez-Caballero, David D, Bieniasz, Paul D PD and Yaravoy, Anton A.. (2003) Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:14519844] |
| 9. Peck, Jeremy W JW, Bowden, Emma T ET and Burbelo, Peter D PD.. (2004) Structure and function of human Vps20 and Snf7 proteins.. The Biochemical journal, (1): [PMID:14583093] |
| 10. Katoh, Keiichi K, Shibata, Hideki H, Hatta, Kazumi K and Maki, Masatoshi M.. (2004) CHMP4b is a major binding partner of the ALG-2-interacting protein Alix among the three CHMP4 isoforms.. Archives of biochemistry and biophysics, (1): [PMID:14678797] |
| 11. Matsuo, Hirotami H and 12 more authors.. (2004) Role of LBPA and Alix in multivesicular liposome formation and endosome organization.. Science (New York, N.Y.), (23): [PMID:14739459] |
| 12. Ichioka, Fumitaka F and 5 more authors.. (2005) Identification of Rab GTPase-activating protein-like protein (RabGAPLP) as a novel Alix/AIP1-interacting protein.. Bioscience, biotechnology, and biochemistry, [PMID:15849434] |
| 13. Segura-Morales, Carolina C and 5 more authors.. (2005) Tsg101 and Alix interact with murine leukemia virus Gag and cooperate with Nedd4 ubiquitin ligases during budding.. The Journal of biological chemistry, (22): [PMID:15908698] |
| 14. Muzny, Donna M DM and 113 more authors.. (2006) The DNA sequence, annotation and analysis of human chromosome 3.. Nature, (27): [PMID:16641997] |
| 15. Yamasaki, Akinori A, Tani, Katsuko K, Yamamoto, Akitsugu A, Kitamura, Naomi N and Komada, Masayuki M.. (2006) The Ca2+-binding protein ALG-2 is recruited to endoplasmic reticulum exit sites by Sec31A and stabilizes the localization of Sec31A.. Molecular biology of the cell, [PMID:16957052] |
| 16. Chi, An A and 16 more authors.. (2006) Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.. Journal of proteome research, [PMID:17081065] |
| 17. Lee, Sangho S, Joshi, Anjali A, Nagashima, Kunio K, Freed, Eric O EO and Hurley, James H JH.. (2007) Structural basis for viral late-domain binding to Alix.. Nature structural & molecular biology, [PMID:17277784] |
| 18. Fisher, Robert D RD and 5 more authors.. (2007) Structural and biochemical studies of ALIX/AIP1 and its role in retrovirus budding.. Cell, (9): [PMID:17350572] |
| 19. Usami, Yoshiko Y, Popov, Sergei S and Göttlinger, Heinrich G HG.. (2007) Potent rescue of human immunodeficiency virus type 1 late domain mutants by ALIX/AIP1 depends on its CHMP4 binding site.. Journal of virology, [PMID:17428861] |
| 20. Carlton, Jez G JG and Martin-Serrano, Juan J.. (2007) Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery.. Science (New York, N.Y.), (29): [PMID:17556548] |
| 21. Morita, Eiji E and 6 more authors.. (2007) Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis.. The EMBO journal, (3): [PMID:17853893] |
| 22. Shibata, Hideki H and 6 more authors.. (2008) Identification of Alix-type and Non-Alix-type ALG-2-binding sites in human phospholipid scramblase 3: differential binding to an alternatively spliced isoform and amino acid-substituted mutants.. The Journal of biological chemistry, (11): [PMID:18256029] |
| 23. McCullough, John J, Fisher, Robert D RD, Whitby, Frank G FG, Sundquist, Wesley I WI and Hill, Christopher P CP.. (2008) ALIX-CHMP4 interactions in the human ESCRT pathway.. Proceedings of the National Academy of Sciences of the United States of America, (3): [PMID:18511562] |
| 24. Carlton, Jez G JG, Agromayor, Monica M and Martin-Serrano, Juan J.. (2008) Differential requirements for Alix and ESCRT-III in cytokinesis and HIV-1 release.. Proceedings of the National Academy of Sciences of the United States of America, (29): [PMID:18641129] |
| 25. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 26. Suzuki, Hironori H and 7 more authors.. (2008) Structural basis for Ca2+ -dependent formation of ALG-2/Alix peptide complex: Ca2+/EF3-driven arginine switch mechanism.. Structure (London, England : 1993), (8): [PMID:18940611] |
| 27. Lee, Hyung Ho HH, Elia, Natalie N, Ghirlando, Rodolfo R, Lippincott-Schwartz, Jennifer J and Hurley, James H JH.. (2008) Midbody targeting of the ESCRT machinery by a noncanonical coiled coil in CEP55.. Science (New York, N.Y.), (24): [PMID:18948538] |
| 28. Okumura, Mayumi M and 6 more authors.. (2009) Penta-EF-hand protein ALG-2 functions as a Ca2+-dependent adaptor that bridges Alix and TSG101.. Biochemical and biophysical research communications, (14): [PMID:19520058] |
| 29. Wang, Pingzhang P, Yu, Peng P, Gao, Peng P, Shi, Taiping T and Ma, Dalong D.. (2009) Discovery of novel human transcript variants by analysis of intronic single-block EST with polyadenylation site.. BMC genomics, (12): [PMID:19906316] |
| 30. Rauch, Susanne S and Martin-Serrano, Juan J.. (2011) Multiple interactions between the ESCRT machinery and arrestin-related proteins: implications for PPXY-dependent budding.. Journal of virology, [PMID:21191027] |
| 31. Benitez, Bruno A BA and 9 more authors.. (2011) Exome-sequencing confirms DNAJC5 mutations as cause of adult neuronal ceroid-lipofuscinosis.. PloS one, [PMID:22073189] |
| 32. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 33. Baietti, Maria Francesca MF and 11 more authors.. (2012) Syndecan-syntenin-ALIX regulates the biogenesis of exosomes.. Nature cell biology, (3): [PMID:22660413] |
| 34. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 35. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315] |
| 36. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 37. Han, Ziying Z and 6 more authors.. (2015) ALIX Rescues Budding of a Double PTAP/PPEY L-Domain Deletion Mutant of Ebola VP40: A Role for ALIX in Ebola Virus Egress.. The Journal of infectious diseases, (1): [PMID:25786915] |
| 38. Khan, Amjad and 6 more authors.. (2020) PDCD6IP, encoding a regulator of the ESCRT complex, is mutated in microcephaly.. Clinical genetics, [PMID:32286682] |
