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Recombinant Amyloid Precursor Protein Antibody - Primary antibody, specific to Amyloid Precursor Protein, Rabbit IgG

ExactAb™
Recombinant
Validated
0.4 mg/mL

Features and benefits

Short Overview:
Recombinant; Rabbit anti Human Amyloid Precursor Protein Antibody; WB, IHC, ICC, IF; Unconjugated
Species reactivity(Reacts with): Human,Mouse,Rat
Isotype: Rabbit IgG

Application:

ICC/IF
IHC
WB

Item Number

Ab088726

Grouped product items
SKU	Size	Availability	Price
Ab088726-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab088726-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$189.90
Ab088726-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab088726-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,599.90

Recombinant; Rabbit anti Human Amyloid Precursor Protein Antibody; WB, IHC, ICC, IF; Unconjugated

View related series

Accession#:P05067 Amyloid Precursor Protein APP Gene ID:351

Basic Description

Product Name	Recombinant Amyloid Precursor Protein Antibody - Primary antibody, specific to Amyloid Precursor Protein, Rabbit IgG
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.4 mg/mL
Host species	Rabbit
Specificity	Amyloid Precursor Protein
Immunogen	A synthetic peptide derived from human Amyloid Precursor Protein (AA 756-770)
Positive Control	WB: HeLa, HEK293 and NIH/3T3 cell lysates. IHC: Human brain tissue. ICC/IF: HeLa cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human Amyloid Precursor Protein Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 IF/ICC: 1/50-1/200 Protein Function: Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Beta-amyloid 42 is a more effective reductant than beta-amyloid 40. Beta-amyloid peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts. Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain. The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis. N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6).

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purification
Form	Liquid
Concentration	0.4 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Amyloid Precursor Protein Antibody (Ab088726) - Western Blot
All lanes: Recombinant Amyloid Precursor Protein Antibody (Ab088726) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 100-140 kDa
Exposure time: 88.0 s

Recombinant Amyloid Precursor Protein Antibody (Ab088726) - IF/ICC
Immunocytochemistry analysis of Amyloid Precursor Protein (green) in HeLa using Recombinant Amyloid Precursor Protein Antibody (Ab088726) and DAPI (blue).

Recombinant Amyloid Precursor Protein Antibody (Ab088726) - IHC
Immunohistochemistry analysis of paraffin-embedded Human brain using Recombinant Amyloid Precursor Protein Antibody (Ab088726). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Dilution info

IF/ICC

1/50 - 1/200

1/500-1/1000

IHC

1/50-1/100

Certificates

Certificate of Analysis(COA)

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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0606654	Certificate of Analysis	Jun 07, 2024	Ab088726
ZJ24F0606653	Certificate of Analysis	Jun 07, 2024	Ab088726

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