ExactAb™, Validated, Recombinant, High performance, 0.144 mg/mL
Host species
Rabbit
Specificity
AR
Immunogen
A synthetic peptide corresponding to residues on the N-terminus of human AR.
Positive Control
WB: MCF7, THP-1 and A549 cell lysates. IHC: Human prostate tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated, 高性能
Product Description
Rabbit anti Human Androgen Receptor Antibody, Recombinant antibody (RR632), could be used for WB and IHC. Application: WB: 1/5000 – 1/10000 IHC: 1/100-1/200 Protein Function Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.144 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Androgen Receptor Antibody (Ab088758) - Western Blot All lanes: Recombinant Androgen Receptor Antibody (Ab088758) at 1/5000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 110 kDa Observed band size: 100 kDa Exposure time: 64.5 s
Recombinant Androgen Receptor Antibody (Ab088758) - Western Blot All lanes: Recombinant Androgen Receptor Antibody (Ab088758) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 110 kDa Observed band size: 100 kDa Exposure time: 100.0 s
Recombinant Androgen Receptor Antibody (Ab088758) - IHC Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling Androgen Receptor with Recombinant Androgen Receptor Antibody (Ab088758) at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.