Recombinant beta IV Tubulin Antibody - Primary antibody, specific to beta IV Tubulin, Rabbit IgG
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.9 mg/mL
Host species
Rabbit
Specificity
beta IV Tubulin
Immunogen
A synthetic peptide derived from human beta IV Tubulin (AA 430-444)
Positive Control
WB: HeLa, K562, Jurkat, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human beta IV Tubulin Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.9 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant beta IV Tubulin Antibody (Ab091172) - Western Blot All lanes: Recombinant beta IV Tubulin Antibody (Ab091172) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 51 kDa Exposure time: 11.6 s
Recombinant beta IV Tubulin Antibody (Ab091172) - IF/ICC Immunocytochemistry analysis of beta IV Tubulin(green) in HeLa using Recombinant beta IV Tubulin Antibody (Ab091172) and DAPI (blue).
Recombinant beta IV Tubulin Antibody (Ab091172) - IHC Immunohistochemistry analysis of paraffin-embedded Human brain using Recombinant beta IV Tubulin Antibody (Ab091172). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.