Recombinant; Rabbit anti Human Bmi1 Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab091398-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab091398-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab091398-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab091398-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,499.90 |
Recombinant; Rabbit anti Human Bmi1 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant Bmi1 Antibody - Primary antibody, specific to Bmi1, Rabbit IgG |
|---|---|
| Synonyms | Polycomb group RING finger protein 4 | RING finger protein 51 | B lymphoma Mo MLV insertion region (mouse) antibody | B lymphoma Mo MLV insertion region 1 homolog antibody | Bmi 1 antibody | BMI1 antibody | BMI1 polycomb ring finger oncogene antibody | BM |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.7 mg/mL |
| Host species | Rabbit |
| Specificity | Bmi1 |
| Immunogen | Recombinant protein of human Bmi1 |
| Positive Control | WB: K562, HeLa, U-2 OS, MCF7, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Bmi1 Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.7 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Bmi1 Antibody (Ab091398) - Western Blot
All lanes: Recombinant Bmi1 Antibody (Ab091398) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 40 kDa
Exposure time: 35.5 s
Recombinant Bmi1 Antibody (Ab091398) - IF/ICC
Immunocytochemistry analysis of BMI1 (green) in HeLa using Recombinant Bmi1 Antibody (Ab091398) and DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| IHC | 1/50 - 1/100 |
| WB |
1/500-1/1000
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0607022 | Certificate of Analysis | Jun 19, 2024 | Ab091398 |
| ZJ24F0607021 | Certificate of Analysis | Jun 19, 2024 | Ab091398 |
| 1. Yang LF, Xing Y, Xiao JX, Xie J, Gao W, Xie J, Wang LT, Wang J, Liu M, Yi Z, Qiu WW.. (2018) Synthesis of Cyanoenone-Modified Diterpenoid Analogs as Novel Bmi-1-Mediated Antitumor Agents.. ACS Med Chem Lett, 9 (11): (1105-1110). [PMID:30429953] |
| 2. Alkema, M J MJ, Wiegant, J J, Raap, A K AK, Berns, A A and van Lohuizen, M M.. (1993) Characterization and chromosomal localization of the human proto-oncogene BMI-1.. Human molecular genetics, [PMID:8268912] |
| 3. Levy, L S LS, Lobelle-Rich, P A PA and Overbaugh, J J.. (1993) flvi-2, a target of retroviral insertional mutagenesis in feline thymic lymphosarcomas, encodes bmi-1.. Oncogene, [PMID:8390036] |
| 4. Gunster, M J MJ and 8 more authors.. (1997) Identification and characterization of interactions between the vertebrate polycomb-group protein BMI1 and human homologs of polyhomeotic.. Molecular and cellular biology, [PMID:9121482] |
| 5. Satijn, D P DP and 9 more authors.. (1997) RING1 is associated with the polycomb group protein complex and acts as a transcriptional repressor.. Molecular and cellular biology, [PMID:9199346] |
| 6. Levine, Stuart S SS and 5 more authors.. (2002) The core of the polycomb repressive complex is compositionally and functionally conserved in flies and humans.. Molecular and cellular biology, [PMID:12167701] |
| 7. Deloukas, P P and 135 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 10.. Nature, (27): [PMID:15164054] |
| 8. Wang, Hengbin H and 6 more authors.. (2004) Role of histone H2A ubiquitination in Polycomb silencing.. Nature, (14): [PMID:15386022] |
| 9. Hernández-Muñoz, Inmaculada I and 9 more authors.. (2005) Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase.. Proceedings of the National Academy of Sciences of the United States of America, (24): [PMID:15897469] |
| 10. Cao, Ru R, Tsukada, Yu-Ichi Y and Zhang, Yi Y.. (2005) Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing.. Molecular cell, (22): [PMID:16359901] |
| 11. Li, Zhizhong Z and 5 more authors.. (2006) Structure of a Bmi-1-Ring1B polycomb group ubiquitin ligase complex.. The Journal of biological chemistry, (21): [PMID:16714294] |
| 12. Maertens, Goedele N GN and 7 more authors.. (2009) Several distinct polycomb complexes regulate and co-localize on the INK4a tumor suppressor locus.. PloS one, (28): [PMID:19636380] |
| 13. Vandamme, Julien J, Völkel, Pamela P, Rosnoblet, Claire C, Le Faou, Perrine P and Angrand, Pierre-Olivier PO.. (2011) Interaction proteomics analysis of polycomb proteins defines distinct PRC1 complexes in mammalian cells.. Molecular & cellular proteomics : MCP, [PMID:21282530] |
| 14. Bentley, Matthew L ML and 5 more authors.. (2011) Recognition of UbcH5c and the nucleosome by the Bmi1/Ring1b ubiquitin ligase complex.. The EMBO journal, (19): [PMID:21772249] |
| 15. McGinty, Robert K; Henrici, Ryan C and Tan, Song.. (2014) Crystal structure of the PRC1 ubiquitylation module bound to the nucleosome.. Nature, (30): [PMID:25355358] |
| 16. Taherbhoy, Asad M AM, Huang, Oscar W OW and Cochran, Andrea G AG.. (2015) BMI1-RING1B is an autoinhibited RING E3 ubiquitin ligase.. Nature communications, (7): [PMID:26151332] |
| 17. Gray, Felicia F and 11 more authors.. (2016) BMI1 regulates PRC1 architecture and activity through homo- and hetero-oligomerization.. Nature communications, (9): [PMID:27827373] |
