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Recombinant CD146 Antibody - Primary antibody, specific to MCAM, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 0.2 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human CD146 Antibody; WB, IHC, IF, ICC; Unconjugated

  • Species reactivity(Reacts with): Human,Rat
  • Isotype: Rabbit IgG
    Application:
  • IF/ICC
  • IHC
  • WB
Item Number
Ab093906
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab093906-10μl
10μl
In stock
$69.90
Ab093906-50μl
50μl
In stock
$219.90
Ab093906-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$369.90
Ab093906-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Recombinant; Rabbit anti Human CD146 Antibody; WB, IHC, IF, ICC; Unconjugated

Basic Description

Product NameRecombinant CD146 Antibody - Primary antibody, specific to MCAM, Rabbit IgG
Specifications & PurityExactAb™, Validated, Recombinant, 0.2 mg/mL
Host speciesRabbit
SpecificityMCAM
ImmunogenA synthetic peptide derived from human CD146 (AA 628-640)
Positive ControlWB: C6, HeLa lysates. IHC: Human colon cancer tissue. IF/ICC: HeLa cells.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human CD146 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/50-1/200
Protein Function:
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen Affinity Purified
FormLiquid
Concentration0.2 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant CD146 Antibody (Ab093906) - Western Blot
All lanes: Recombinant CD146 Antibody (Ab093906) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 72 kDa
Observed band size: 111 kDa
Exposure time: 8.0 s

Recombinant CD146 Antibody (Ab093906) - IF/ICC
Immunocytochemistry analysis of CD146 (green) in HeLa using Recombinant CD146 Antibody (Ab093906), and cells were counterstained with DAPI (blue).

Recombinant CD146 Antibody (Ab093906) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using Recombinant CD146 Antibody (Ab093906). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets

MCAM Tbio Cell surface glycoprotein MUC18 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

IHC

1/50 - 1/100

IF/ICC

1/50 - 1/200

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0506017Certificate of AnalysisMay 23, 2024 Ab093906
ZJ24F0506018Certificate of AnalysisMay 23, 2024 Ab093906

Related Documents

Solution Calculators