A synthetic peptide derived from human CD1c (AA 100-169)
Positive Control
WB: Jurkat and MOLT-4 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human CD1c Antibody, Recombinant, could be used for WB and so on. Application WB: 1/500-1/1000 Protein Function Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.8 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant CD1c Antibody (Ab094135) - Western Blot All lanes: Recombinant CD1c Antibody (Ab094135) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 40 kDa Exposure time: 50.0 s
1.Martin, L H LH, Calabi, F F, Lefebvre, F A FA, Bilsland, C A CA and Milstein, C C.. (1987) Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2447586]
2.Aruffo, A A and Seed, B B.. (1989) Expression of cDNA clones encoding the thymocyte antigens CD1a, b, c demonstrates a hierarchy of exclusion in fibroblasts.. Journal of immunology (Baltimore, Md. : 1950), (1):[PMID:2701945]
3.Martin, L H LH, Calabi, F F and Milstein, C C.. (1986) Isolation of CD1 genes: a family of major histocompatibility complex-related differentiation antigens.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3097645]
4.Han, M M, Hannick, L I LI, DiBrino, M M and Robinson, M A MA.. (1999) Polymorphism of human CD1 genes.. Tissue antigens, [PMID:10488738]
5.Moody, D B DB and 10 more authors.. (2000) CD1c-mediated T-cell recognition of isoprenoid glycolipids in Mycobacterium tuberculosis infection.. Nature, (20):[PMID:10786796]
6.Sugita, M M, van Der Wel, N N, Rogers, R A RA, Peters, P J PJ and Brenner, M B MB.. (2000) CD1c molecules broadly survey the endocytic system.. Proceedings of the National Academy of Sciences of the United States of America, (18):[PMID:10890914]
7.Briken, V V, Jackman, R M RM, Watts, G F GF, Rogers, R A RA and Porcelli, S A SA.. (2000) Human CD1b and CD1c isoforms survey different intracellular compartments for the presentation of microbial lipid antigens.. The Journal of experimental medicine, (17):[PMID:10899914]
8.Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18):[PMID:16710414]
9.Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973]
10.Scharf, Louise L and 14 more authors.. (2010) The 2.5 Å structure of CD1c in complex with a mycobacterial lipid reveals an open groove ideally suited for diverse antigen presentation.. Immunity, (14):[PMID:21167756]
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