Recombinant; Rabbit anti Human CD73 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab095249-10μl | 10μl | In stock | $69.90 | |
| Ab095249-50μl | 50μl | In stock | $199.90 | |
| Ab095249-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab095249-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human CD73 Antibody; WB; Unconjugated
| Product Name | Recombinant CD73 Antibody - Primary antibody, specific to CD73, Rabbit IgG |
|---|---|
| Synonyms | 5'-NT | EC 3.1.3.5 | Ecto-5'-nucleotidase | CALJA | NT5 | 5' nucleotidase (CD73) | 5'-NT | 5-NT | 5'-Nucleotidase | 5'-nucleotidase, ecto (CD73) | CD73 antigen | CD73 | E5NT | EC 3.1.3.5 | ecto-5'-nucleotidase | eN | eNT | NT | NT55'-nucleotidase | NT5E | |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 1.51 mg/mL |
| Host species | Rabbit |
| Specificity | CD73 |
| Immunogen | A synthetic peptide derived from human CD73 (AA 500-574) |
| Positive Control | WB: HT-29 and Hep G2 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human CD73 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.51 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant CD73 Antibody (Ab095249) - Western Blot
All lanes: Recombinant CD73 Antibody (Ab095249) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 63 kDa
Observed band size: 72 kDa
Exposure time: 148.8 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505474 | Certificate of Analysis | May 13, 2024 | Ab095249 |
| ZJ24F0505473 | Certificate of Analysis | May 13, 2024 | Ab095249 |
| 1. Geoghegan JC, Diedrich G, Lu X, Rosenthal K, Sachsenmeier KF, Wu H, Dall'Acqua WF, Damschroder MM. (2016) Inhibition of CD73 AMP hydrolysis by a therapeutic antibody with a dual, non-competitive mechanism of action.. MAbs, 8 (3): (454-67). [PMID:26854859] |
| 2. Eliahu S, Lecka J, Reiser G, Haas M, Bigonnesse F, Lévesque SA, Pelletier J, Sévigny J, Fischer B.. (2010) Diadenosine 5',5''-(boranated)polyphosphonate analogues as selective nucleotide pyrophosphatase/phosphodiesterase inhibitors.. J Med Chem, 53 (24): (8485-8497). [PMID:21090681] |
| 3. al-Rashida M, Raza R, Abbas G, Shah MS, Kostakis GE, Lecka J, Sévigny J, Muddassar M, Papatriantafyllopoulou C, Iqbal J.. (2013) Identification of novel chromone based sulfonamides as highly potent and selective inhibitors of alkaline phosphatases.. Eur J Med Chem, 66 (438-449). [PMID:23831808] |
| 4. Gillerman I, Lecka J, Simhaev L, Munkonda MN, Fausther M, Martín-Satué M, Senderowitz H, Sévigny J, Fischer B.. (2014) 2-Hexylthio-β,γ-CH2-ATP is an effective and selective NTPDase2 inhibitor.. J Med Chem, 57 (14): (5919-5934). [PMID:24972256] |
| 5. Lee SY, Perotti A, De Jonghe S, Herdewijn P, Hanck T, Müller CE.. (2016) Thiazolo[3,2-a]benzimidazol-3(2H)-one derivatives: Structure-activity relationships of selective nucleotide pyrophosphatase/phosphodiesterase1 (NPP1) inhibitors.. Bioorg Med Chem, 24 (14): (3157-3165). [PMID:27265686] |
| 6. Abdel-Magid AF.. (2017) Inhibitors of CD73 May Provide a Treatment for Cancer and Autoimmune Diseases.. ACS Med Chem Lett, 8 (8): (781-782). [PMID:28835785] |
| 7. Junker A, Renn C, Dobelmann C, Namasivayam V, Jain S, Losenkova K, Irjala H, Duca S, Balasubramanian R, Chakraborty S, Börgel F, Zimmermann H, Yegutkin GG, Müller CE, Jacobson KA.. (2019) Structure-Activity Relationship of Purine and Pyrimidine Nucleotides as Ecto-5'-Nucleotidase (CD73) Inhibitors.. J Med Chem, 62 (7): (3677-3695). [PMID:30895781] |
| 8. Du X,Moore J,Blank BR,Eksterowicz J,Sutimantanapi D,Yuen N,Metzger T,Chan B,Huang T,Chen X,Chen Y,Duong F,Kong W,Chang JH,Sun J,Zavorotinskaya T,Ye Q,Junttila MR,Ndubaku C,Friedman LS,Fantin VR,Sun D. (2020) Orally Bioavailable Small-Molecule CD73 Inhibitor (OP-5244) Reverses Immunosuppression through Blockade of Adenosine Production.. J Med Chem, 63 (18): (10433-10459). [PMID:32865411] |
| 9. Misumi, Y Y, Ogata, S S, Ohkubo, K K, Hirose, S S and Ikehara, Y Y.. (1990) Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form.. European journal of biochemistry, (17): [PMID:2129526] |
| 10. Klemens, M R MR and 5 more authors.. (1990) Characterization of soluble vs membrane-bound human placental 5'-nucleotidase.. Biochemical and biophysical research communications, (15): [PMID:2173922] |
| 11. Hansen, K R KR, Resta, R R, Webb, C F CF and Thompson, L F LF.. (1995) Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene.. Gene, (29): [PMID:8566797] |
| 12. Otsuki, Tetsuji T and 24 more authors.. (2005) Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.. DNA research : an international journal for rapid publication of reports on genes and genomes, [PMID:16303743] |
| 13. Ustunsoy, Hasim H and 5 more authors.. () The effects of pentoxifylline on the myocardial inflammation and ischemia-reperfusion injury during cardiopulmonary bypass.. Journal of cardiac surgery, [PMID:16426349] |
| 14. Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973] |
| 15. St Hilaire, Cynthia C and 20 more authors.. (2011) NT5E mutations and arterial calcifications.. The New England journal of medicine, (3): [PMID:21288095] |
| 16. Garavaglia, Silvia S and 9 more authors.. (2012) The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism.. The Biochemical journal, (1): [PMID:21933152] |
| 17. Heuts, Dominic P H M DP and 6 more authors.. (2012) Crystal structure of a soluble form of human CD73 with ecto-5'-nucleotidase activity.. Chembiochem : a European journal of chemical biology, (5): [PMID:22997138] |
| 18. Knapp, Karen K and 5 more authors.. (2012) Crystal structure of the human ecto-5'-nucleotidase (CD73): insights into the regulation of purinergic signaling.. Structure (London, England : 1993), (5): [PMID:23142347] |
| 19. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 20. Fausther, Michel M, Lavoie, Elise G EG, Goree, Jessica R JR, Baldini, Giulia G and Dranoff, Jonathan A JA.. (2014) NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein.. PloS one, [PMID:24887587] |
| 21. Channar, Pervaiz Ali PA and 8 more authors.. (2017) Isonicotinohydrazones as inhibitors of alkaline phosphatase and ecto-5'-nucleotidase.. Chemical biology & drug design, [PMID:27589035] |
