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Recombinant CDT2/RAMP Antibody - Primary antibody, specific to DTL, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 1.3 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human CDT2/RAMP Antibody; WB, IHC; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IHC
  • WB
Item Number
Ab095882
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab095882-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$79.90
Ab095882-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$229.90
Ab095882-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$379.90
Ab095882-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Recombinant; Rabbit anti Human CDT2/RAMP Antibody; WB, IHC; Unconjugated

Basic Description

Product NameRecombinant CDT2/RAMP Antibody - Primary antibody, specific to DTL, Rabbit IgG
Specifications & PurityExactAb™, Validated, Recombinant, 1.3 mg/mL
Host speciesRabbit
SpecificityDTL
ImmunogenRecombinant protein of human CDT2
Positive ControlWB: HeLa, HEK293, K562, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human CDT2/RAMP Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB:1/500-1/1000
IHC: 1/50-1/100
Protein Function:
Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen Affinity Purified
FormLiquid
Concentration1.3 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant CDT2/RAMP Antibody (Ab095882) - Western Blot
All lanes: Recombinant CDT2/RAMP Antibody (Ab095882) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 79 kDa
Observed band size: 95 kDa
Exposure time: 30.0 s

Recombinant CDT2/RAMP Antibody (Ab095882) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant CDT2/RAMP Antibody (Ab095882). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets

DTL Tbio Denticleless protein homolog 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
IHC

1/50 - 1/100

WB

1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0708925Certificate of AnalysisJul 29, 2024 Ab095882
ZJ24F0708924Certificate of AnalysisJul 29, 2024 Ab095882

Related Documents

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