Recombinant CDT2/RAMP Antibody - Primary antibody, specific to DTL, Rabbit IgG
Specifications & Purity
ExactAb™, Validated, Recombinant, 1.3 mg/mL
Host species
Rabbit
Specificity
DTL
Immunogen
Recombinant protein of human CDT2
Positive Control
WB: HeLa, HEK293, K562, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human CDT2/RAMP Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB:1/500-1/1000 IHC: 1/50-1/100 Protein Function: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen Affinity Purified
Form
Liquid
Concentration
1.3 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant CDT2/RAMP Antibody (Ab095882) - Western Blot All lanes: Recombinant CDT2/RAMP Antibody (Ab095882) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 79 kDa Observed band size: 95 kDa Exposure time: 30.0 s
Recombinant CDT2/RAMP Antibody (Ab095882) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant CDT2/RAMP Antibody (Ab095882). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.